Your browser doesn't support javascript.
Severely ill COVID-19 patients display augmented functional properties in SARS-CoV-2-reactive CD8 + T cells.
Kusnadi, Anthony; Ramírez-Suástegui, Ciro; Fajardo, Vicente; Chee, Serena J; Meckiff, Benjamin J; Simon, Hayley; Pelosi, Emanuela; Seumois, Grégory; Ay, Ferhat; Vijayanand, Pandurangan; Ottensmeier, Christian H.
  • Kusnadi A; La Jolla Institute for Immunology, La Jolla, CA, USA.
  • Ramírez-Suástegui C; These authors jointly contributed to the work.
  • Fajardo V; La Jolla Institute for Immunology, La Jolla, CA, USA.
  • Chee SJ; These authors jointly contributed to the work.
  • Meckiff BJ; La Jolla Institute for Immunology, La Jolla, CA, USA.
  • Simon H; These authors jointly contributed to the work.
  • Pelosi E; NIHR and CRUK Southampton Experimental Cancer Medicine Center, Faculty of Medicine, University of Southampton, Southampton, UK.
  • Seumois G; These authors jointly contributed to the work.
  • Ay F; La Jolla Institute for Immunology, La Jolla, CA, USA.
  • Vijayanand P; La Jolla Institute for Immunology, La Jolla, CA, USA.
  • Ottensmeier CH; Southampton Specialist Virology Centre, Department of Infection, University Hospital Southampton NHS Foundation Trust, Southampton, UK.
bioRxiv ; 2020 Jul 10.
Article in English | MEDLINE | ID: covidwho-664327
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
The molecular properties of CD8 + T cells that respond to SARS-CoV-2 infection are not fully known. Here, we report on the single-cell transcriptomes of >80,000 virus-reactive CD8 + T cells from 39 COVID-19 patients and 10 healthy subjects. COVID-19 patients segregated into two groups based on whether the dominant CD8 + T cell response to SARS-CoV-2 was 'exhausted' or not. SARS-CoV-2-reactive cells in the exhausted subset were increased in frequency and displayed lesser cytotoxicity and inflammatory features in COVID-19 patients with mild compared to severe illness. In contrast, SARS-CoV-2-reactive cells in the non-exhausted subsets from patients with severe disease showed enrichment of transcripts linked to co-stimulation, pro-survival NF-κB signaling, and anti-apoptotic pathways, suggesting the generation of robust CD8 + T cell memory responses in patients with severe COVID-19 illness. CD8 + T cells reactive to influenza and respiratory syncytial virus from healthy subjects displayed polyfunctional features. Cells with such features were mostly absent in SARS-CoV-2 responsive cells from both COVID-19 patients and healthy controls non-exposed to SARS-CoV-2. Overall, our single-cell analysis revealed substantial diversity in the nature of CD8 + T cells responding to SARS-CoV-2.

Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Language: English Year: 2020 Document Type: Article Affiliation country: 2020.07.09.194027

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Language: English Year: 2020 Document Type: Article Affiliation country: 2020.07.09.194027