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[Study on therapeutic effect of Chaiyin Particles on combining disease with syndrome of human coronavirus pneumonia with pestilence attacking lung syndrome based on regulation of immune function].
Bao, Yan-Yan; Shi, Yu-Jing; Guo, Shan-Shan; Geng, Zi-Han; Gao, Ying-Jie; Bao, Lei; Zhao, Rong-Hua; Sun, Jing; Zhang, Gui-Min; Guan, Yong-Xia; Cui, Xiao-Lan.
  • Bao YY; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Shi YJ; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Guo SS; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Geng ZH; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Gao YJ; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Bao L; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Zhao RH; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Sun J; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Zhang GM; Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China State Key Laboratory of Generic Manufacture Technology of Traditional Chinese Medicine Linyi 276006, China.
  • Guan YX; Lunan Pharmaceutical Group Co., Ltd. Linyi 276006, China State Key Laboratory of Generic Manufacture Technology of Traditional Chinese Medicine Linyi 276006, China.
  • Cui XL; Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi ; 45(13): 3020-3027, 2020 Jul.
Article in Chinese | MEDLINE | ID: covidwho-679282
ABSTRACT
According to the classification of traditional Chinese medicine syndromes of coronavirus disease 2019 by the national competent authority, this study determined that human coronavirus 229 E(HCoV-229 E) was infected in a mouse model of cold and dampness syndrome, so as to build the human coronavirus pneumonia with pestilence attacking lung syndrome model. The model can simulate the traditional Chinese medicine treatment of common disease syndromes in Coronavirus Disease 2019 Diagnosis and Treatment Program(the sixth edition for trial). Specific steps were as follows. ABALB/c mouse model of cold and dampness syndrome was established, based on which, HCoV-229 E virus was infected; then the experiment was divided into normal control group, infection control group, cold-dampness control group, cold-dampness infection group(the model group), high-dose Chaiyin Particles group(8.8 g·kg~(-1)·d~(-1)), and low-dose Chaiyin Particles group(4.4 g·kg~(-1)·d~(-1)). On the day of infection, Chaiyin Particles was given for three consecutive days. Lung tissues were collected the day after the last dose, and the lung index and inhibition rate were calculated. The nucleic acid of lung tissue was extracted, and the HCoV-229 E virus load was detected by Real-time fluorescent quantitative RT-PCR. Blood leukocytes were separated, and the percentage of T and B lymphocytes was detected by flow cytometry. Lung tissue protein was extracted, and IL-6, IL-10, TNF-α and IFN-γ contents were detected by ELISA. High and low-dose Chaiyin Particles significantly reduced the lung index(P<0.01) of mice of human coronavirus pneumonia with pestilence attacking the lung syndrome, and the inhibition rates were 61.02% and 55.45%, respectively. Compared with the model control group, high and low-dose Chaiyin Particles significantly increased cross blood CD4~+ T lymphocytes, CD8~+T lymphocytes and total B lymphocyte percentage(P<0.05, P<0.01), and reduced IL-10, TNF-α and IFN-γ levels in lungs(P<0.01). In vitro results showed that TC_(50), TC_0, IC_(50) and TI of Chaiyin Particles were 4.46 mg·mL~(-1), 3.13 mg·mL~(-1), 1.12 mg·mL~(-1) and 4. The control group of in vitro culture cells had no HCoV-229 E virus nucleic acid expression. The expression of HCoV-229 E virus nucleic acid in the virus control group was 1.48×10~7 copies/mL, and Chaiyin Particles significantly reduced HCoV-229 E expression at doses of 3.13 and 1.56 mg·mL~(-1), and the expression of HCoV-229 E nucleic acid was 9.47×10~5 and 9.47×10~6 copies/mL, respectively. Chaiyin Particles has a better effect on the mouse model with human coronavirus pneumonia with pestilence attacking the lung syndrome, and could play a role by enhancing immunity, and reducing inflammatory factor expression.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Drugs, Chinese Herbal / Coronavirus Infections / Coronavirus 229E, Human Type of study: Experimental Studies / Randomized controlled trials Topics: Traditional medicine Limits: Animals / Humans Language: Chinese Journal: Zhongguo Zhong Yao Za Zhi Journal subject: Pharmacology / Complementary Therapies Year: 2020 Document Type: Article Affiliation country: J.cnki.cjcmm.20200419.401

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Drugs, Chinese Herbal / Coronavirus Infections / Coronavirus 229E, Human Type of study: Experimental Studies / Randomized controlled trials Topics: Traditional medicine Limits: Animals / Humans Language: Chinese Journal: Zhongguo Zhong Yao Za Zhi Journal subject: Pharmacology / Complementary Therapies Year: 2020 Document Type: Article Affiliation country: J.cnki.cjcmm.20200419.401