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DNA nanoscaffold-based SARS-CoV-2 detection for COVID-19 diagnosis.
Jiao, Jin; Duan, Chengjie; Xue, Lan; Liu, Yunfei; Sun, Weihao; Xiang, Yang.
  • Jiao J; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.
  • Duan C; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.
  • Xue L; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.
  • Liu Y; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China.
  • Sun W; Department of Geriatric Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210023, PR China. Electronic address: swh@njmu.edu.cn.
  • Xiang Y; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China. Electronic address: xiangy@nju.edu.cn.
Biosens Bioelectron ; 167: 112479, 2020 Nov 01.
Article in English | MEDLINE | ID: covidwho-684409
ABSTRACT
COVID-19 pandemic outbreak is the most astounding scene ever experienced in the 21st century. It has been determined to be caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With the global pandemic, the lack of efficient rapid and accurate molecular diagnostic testing tools has hindered the public opportunely response to the emerging viral threat. Herein, a DNA nanoscaffold hybrid chain reaction (DNHCR)-based nucleic acid assay strategy is reported for rapid detection of SARS-CoV-2 RNA. In this method, the DNA nanoscaffolds have been first constructed by the self-assembly of long DNA strands and self-quenching probes (H1). Then, the SARS-CoV-2 RNA will initiate the hybridization of H1 and free H2 DNA probes along the nanoscaffold, and an illuminated DNA nanostring is instantly obtained. By taking advantages of the localization design of the H1 probes and the temperature tolerance of the isothermal amplification, the proposed DNHCR method can detect target at short responding time (within 10 min) and mild condition (15 °C-35 °C). Moreover, the reliability of DNHCR method in serum and saliva samples have also been validated. Therefore, DNHCR-based method is expected to provide a simple and faster alternative to the traditional SARS-CoV-2 qRT-PCR assay.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Biosensing Techniques / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Type of study: Diagnostic study / Observational study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Biosensing Techniques / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Type of study: Diagnostic study / Observational study / Prognostic study Topics: Long Covid Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2020 Document Type: Article