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Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed.
Mayer, Florian J; Ratzinger, Franz; Schmidt, Ralf L J; Greiner, Georg; Landt, Olfert; Am Ende, Alexander; Corman, Victor M; Perkmann-Nagele, Nicole; Watkins-Riedel, Thomas; Petermann, Dagmar; Abadir, Karoline; Zweimüller-Mayer, Josef; Strassl, Robert.
  • Mayer FJ; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
  • Ratzinger F; Ihr Labor, Medical Diagnostic Laboratories , Vienna, Austria.
  • Schmidt RLJ; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
  • Greiner G; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
  • Landt O; Tib-Molbiol , Berlin, Germany.
  • Am Ende A; Tib-Molbiol , Berlin, Germany.
  • Corman VM; Charité - Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF) , Berlin, Germany.
  • Perkmann-Nagele N; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
  • Watkins-Riedel T; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
  • Petermann D; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
  • Abadir K; QIAGEN GmbH , Vienna, Austria.
  • Zweimüller-Mayer J; QIAGEN GmbH , Vienna, Austria.
  • Strassl R; Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna , Vienna, Austria.
Virulence ; 11(1): 964-967, 2020 12.
Article in English | MEDLINE | ID: covidwho-690823
ABSTRACT
Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / High-Throughput Nucleotide Sequencing / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: Virulence Year: 2020 Document Type: Article Affiliation country: 21505594.2020.1798041

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / High-Throughput Nucleotide Sequencing / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: Virulence Year: 2020 Document Type: Article Affiliation country: 21505594.2020.1798041