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A Rapid, Simple, Inexpensive, and Mobile Colorimetric Assay COVID-19-LAMP for Mass On-Site Screening of COVID-19.
Chow, Franklin Wang-Ngai; Chan, Tony Tat-Yin; Tam, Anthony Raymond; Zhao, Suhui; Yao, Weiming; Fung, Joshua; Cheng, Flora Ka-Kei; Lo, George Chi-Shing; Chu, Stella; Aw-Yong, Kam Leng; Tang, James Yat-Man; Tsang, Chi-Ching; Luk, Hayes Kam-Hei; Wong, Antonio Cheuk-Pui; Li, Kenneth Sze-Ming; Zhu, Longchao; He, Zirong; Tam, Emily Wan Ting; Chung, Tom Wai-Hin; Wong, Sally Cheuk Ying; Que, Tak-Lun; Fung, Kitty Sau-Chun; Lung, David Christopher; Wu, Alan Ka-Lun; Hung, Ivan Fan-Ngai; Woo, Patrick Chiu-Yat; Lau, Susanna Kar-Pui.
  • Chow FW; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Chan TT; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Tam AR; Department of Medicine, Queen Mary Hospital, Hong Kong 102 Pok Fu Lam Road, Pok Fu Lam, Hong Kong Island, Hong Kong.
  • Zhao S; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Yao W; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Fung J; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Cheng FK; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Lo GC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Chu S; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Aw-Yong KL; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Tang JY; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Tsang CC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Luk HK; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Wong AC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Li KS; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Zhu L; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • He Z; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Tam EWT; Department of Food and Health Sciences, Faculty of Science and Technology, Technological and Higher Education Institute of Hong Kong, Hong Kong Tsing Yi Road, Tsing Yi, Hong Kong.
  • Chung TW; Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Wong SCY; Department of Pathology, Queen Elizabeth Hospital, Hong Kong 10/F, Block M, Queen Elizabeth Hospital, 30 Gascoigne Road, Hong Kong.
  • Que TL; Department of Pathology, Tuen Mun Hospital, Hong Kong Pathology Block, 23 Tsing Chung Koon Road, Tuen Mun, New Territories, Hong Kong.
  • Fung KS; Department of Pathology, United Christian Hospital, Hong Kong 130 Hip Wo Street, Kwun Tong, Hong Kong.
  • Lung DC; Department of Pathology, Queen Elizabeth Hospital, Hong Kong 10/F, Block M, Queen Elizabeth Hospital, 30 Gascoigne Road, Hong Kong.
  • Wu AK; Department of Clinical Pathology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 3 Lok Man Road, Chai Wan, Hong Kong.
  • Hung IF; Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 4/F, Professorial Block, Queen Mary Hospital, 102 Pok Fu Lam Road, Hong Kong.
  • Woo PC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 19/F, Block T, Queen Mary Hospital, 102 Pokfulam Road, Hong Kong.
  • Lau SK; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Laboratory Block, 21 Sassoon Road, Pokfulam and University Pathology Building, Queen Mary Hospital, The University of Hong Kong, Hong Kong.
Int J Mol Sci ; 21(15)2020 Jul 29.
Article in English | MEDLINE | ID: covidwho-693630
ABSTRACT
To control the COVID-19 pandemic and prevent its resurgence in areas preparing for a return of economic activities, a method for a rapid, simple, and inexpensive point-of-care diagnosis and mass screening is urgently needed. We developed and evaluated a one-step colorimetric reverse-transcriptional loop-mediated isothermal amplification assay (COVID-19-LAMP) for detection of SARS-CoV-2, using SARS-CoV-2 isolate and respiratory samples from patients with COVID-19 (n = 223) and other respiratory virus infections (n = 143). The assay involves simple equipment and techniques and low cost, without the need for expensive qPCR machines, and the result, indicated by color change, is easily interpreted by naked eyes. COVID-19-LAMP can detect SARS-CoV-2 RNA with detection limit of 42 copies/reaction. Of 223 respiratory samples positive for SARS-CoV-2 by qRT-PCR, 212 and 219 were positive by COVID-19-LAMP at 60 and 90 min (sensitivities of 95.07% and 98.21%) respectively, with the highest sensitivities among nasopharyngeal swabs (96.88% and 98.96%), compared to sputum/deep throat saliva samples (94.03% and 97.02%), and throat swab samples (93.33% and 98.33%). None of the 143 samples with other respiratory viruses were positive by COVID-19-LAMP, showing 100% specificity. Samples with higher viral load showed shorter detection time, some as early as 30 min. This inexpensive, highly sensitive and specific COVID-19-LAMP assay can be useful for rapid deployment as mobile diagnostic units to resource-limiting areas for point-of-care diagnosis, and for unlimited high-throughput mass screening at borders to reduce cross-regional transmission.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Mass Screening / Coronavirus Infections / Colorimetry / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Year: 2020 Document Type: Article Affiliation country: Ijms21155380

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Mass Screening / Coronavirus Infections / Colorimetry / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Year: 2020 Document Type: Article Affiliation country: Ijms21155380