Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
Arch Virol
; 165(10): 2373-2377, 2020 Oct.
Article
in English
| MEDLINE | ID: covidwho-695405
ABSTRACT
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
RNA, Viral
/
Coronavirus Infections
/
Clinical Laboratory Techniques
/
Betacoronavirus
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Arch Virol
Year:
2020
Document Type:
Article
Affiliation country:
S00705-020-04737-w
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