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A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
Kim, Dae-Kyum; Knapp, Jennifer J; Kuang, Da; Chawla, Aditya; Cassonnet, Patricia; Lee, Hunsang; Sheykhkarimli, Dayag; Samavarchi-Tehrani, Payman; Abdouni, Hala; Rayhan, Ashyad; Li, Roujia; Pogoutse, Oxana; Coyaud, Étienne; van der Werf, Sylvie; Demeret, Caroline; Gingras, Anne-Claude; Taipale, Mikko; Raught, Brian; Jacob, Yves; Roth, Frederick P.
  • Kim DK; Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
  • Knapp JJ; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
  • Kuang D; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.
  • Chawla A; Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
  • Cassonnet P; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
  • Lee H; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.
  • Sheykhkarimli D; Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
  • Samavarchi-Tehrani P; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
  • Abdouni H; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.
  • Rayhan A; Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
  • Li R; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
  • Pogoutse O; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.
  • Coyaud É; Unité de Génétique Moléculaire des Virus à ARN, Département Virologie, Institut Pasteur, Paris, France.
  • van der Werf S; UMR3569, Centre National de la Recherche Scientifique, Paris, France.
  • Demeret C; Université de Paris, Paris, France.
  • Gingras AC; Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
  • Taipale M; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
  • Raught B; Donnelly Centre, University of Toronto, Toronto, Ontario, Canada.
  • Jacob Y; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
  • Roth FP; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada.
G3 (Bethesda) ; 10(9): 3399-3402, 2020 09 02.
Article in English | MEDLINE | ID: covidwho-695997
ABSTRACT
The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Open Reading Frames / Betacoronavirus Limits: Humans Language: English Journal: G3 (Bethesda) Year: 2020 Document Type: Article Affiliation country: G3.120.401554

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Open Reading Frames / Betacoronavirus Limits: Humans Language: English Journal: G3 (Bethesda) Year: 2020 Document Type: Article Affiliation country: G3.120.401554