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Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses.
Schmidt, Fabian; Weisblum, Yiska; Muecksch, Frauke; Hoffmann, Hans-Heinrich; Michailidis, Eleftherios; Lorenzi, Julio C C; Mendoza, Pilar; Rutkowska, Magdalena; Bednarski, Eva; Gaebler, Christian; Agudelo, Marianna; Cho, Alice; Wang, Zijun; Gazumyan, Anna; Cipolla, Melissa; Caskey, Marina; Robbiani, Davide F; Nussenzweig, Michel C; Rice, Charles M; Hatziioannou, Theodora; Bieniasz, Paul D.
  • Schmidt F; Laboratory of Retrovirology, The Rockefeller University, New York, NY.
  • Weisblum Y; Laboratory of Retrovirology, The Rockefeller University, New York, NY.
  • Muecksch F; Laboratory of Retrovirology, The Rockefeller University, New York, NY.
  • Hoffmann HH; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY.
  • Michailidis E; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY.
  • Lorenzi JCC; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Mendoza P; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Rutkowska M; Laboratory of Retrovirology, The Rockefeller University, New York, NY.
  • Bednarski E; Laboratory of Retrovirology, The Rockefeller University, New York, NY.
  • Gaebler C; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Agudelo M; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Cho A; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Wang Z; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Gazumyan A; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Cipolla M; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Caskey M; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Robbiani DF; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Nussenzweig MC; Institute for Research in Biomedicine, Università della Svizzera italiana, Bellinzona, Switzerland.
  • Rice CM; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY.
  • Hatziioannou T; Howard Hughes Medical Institute, The Rockefeller University, New York, NY.
  • Bieniasz PD; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY.
J Exp Med ; 217(11)2020 11 02.
Article in English | MEDLINE | ID: covidwho-697830
Preprint
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ABSTRACT
The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARS-CoV-2-specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1), and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoassay / Coronavirus Infections / Antibodies, Neutralizing / Betacoronavirus / Antibodies, Viral Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoassay / Coronavirus Infections / Antibodies, Neutralizing / Betacoronavirus / Antibodies, Viral Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Year: 2020 Document Type: Article