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Gene Cloning and Sequence Analysis of Angiotensin Converting Enzyme 2 (ACE2) in China Sheldrake Duck
Acta Veterinaria et Zootechnica Sinica ; 51(7):1597-1606, 2020.
Article in Chinese | EMBASE | ID: covidwho-701915
ABSTRACT
Since the discovery of angiotensin-converting enzyme 2 (ACE2),its pathophysiological functions,especially as functional receptors for coronaviruses such as SARS and COVID-19,have shown great potential.To clarify the gene sequence and structure of different species can provide a basis for the study of the mechanism of coronavirus infection. In this experiment,RT-PCR and Western blot were firstly used to detect the presence of ACE2 in different tissues of China Sheldrake duck.Then the homologous cloning and PCR technology were used to amplify the complete ORF sequence of the China Sheldrake duck ACE2 gene,and then TA cloned into pMD-19T.The vector was sequenced,and the obtained sequence was analyzed by bioinformatics. The expression of ACE2 gene and protein in heart,liver,lung,kidney and other tissues was confirmed.Gene cloning results showed that the full-length CDS sequence of the China Sheldrake duck ACE2 gene was 2 435 bp,encoding 805 amino acid residues,and its nucleotide sequence and amino acid sequence homology with human ACE2 were 66.2% and 66.4%,respectively,and on different branches of the evolutionary tree.Analysis of the 18 key amino acid residues related to the binding of the SARS virus S protein in humans found that except for the 330th and 353th amino acids, the rest were different from humans. Structural analysis revealed that the duck ACE2 was a type I transmembrane protein with multiple N-glycosylation sites.The study obtained the complete ORF sequence and related basic data of the ACE2 gene of China Sheldrake duck for the first time. The obtained sequence had been uploaded to GenBank and successfully included.The results provided a theoretical basis for the functional study of ACE2 on ducks.

Full text: Available Collection: Databases of international organizations Database: EMBASE Language: Chinese Journal: Acta Veterinaria et Zootechnica Sinica Year: 2020 Document Type: Article

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Full text: Available Collection: Databases of international organizations Database: EMBASE Language: Chinese Journal: Acta Veterinaria et Zootechnica Sinica Year: 2020 Document Type: Article