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Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction.
Israeli, Ofir; Beth-Din, Adi; Paran, Nir; Stein, Dana; Lazar, Shirley; Weiss, Shay; Milrot, Elad; Atiya-Nasagi, Yafit; Yitzhaki, Shmuel; Laskar, Orly; Schuster, Ofir.
  • Israeli O; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Beth-Din A; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Paran N; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Stein D; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Lazar S; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Weiss S; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Milrot E; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Atiya-Nasagi Y; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Yitzhaki S; Israel Institute for Biological Research, Ness Ziona, Israel.
  • Laskar O; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.
  • Schuster O; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.
Int J Infect Dis ; 99: 352-354, 2020 Oct.
Article in English | MEDLINE | ID: covidwho-703985
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ABSTRACT
The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharyngeal SARS-CoV-2 swabs. The findings showed an advantage for the extraction procedure; however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Animals / Humans Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: J.ijid.2020.08.015

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Coronavirus Infections / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Animals / Humans Language: English Journal: Int J Infect Dis Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: J.ijid.2020.08.015