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Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients.
Zhu, Linnan; Yang, Penghui; Zhao, Yingze; Zhuang, Zhenkun; Wang, Zhifeng; Song, Rui; Zhang, Jie; Liu, Chuanyu; Gao, Qianqian; Xu, Qumiao; Wei, Xiaoyu; Sun, Hai-Xi; Ye, Beiwei; Wu, Yanan; Zhang, Ning; Lei, Guanglin; Yu, Lingxiang; Yan, Jin; Diao, Guanghao; Meng, Fanping; Bai, Changqing; Mao, Panyong; Yu, Yeya; Wang, Mingyue; Yuan, Yue; Deng, Qiuting; Li, Ziyi; Huang, Yunting; Hu, Guohai; Liu, Yang; Wang, Xiaoqian; Xu, Ziqian; Liu, Peipei; Bi, Yuhai; Shi, Yi; Zhang, Shaogeng; Chen, Zhihai; Wang, Jian; Xu, Xun; Wu, Guizhen; Wang, Fu-Sheng; Gao, George F; Liu, Longqi; Liu, William J.
  • Zhu L; BGI-Shenzhen, 518103 Shenzhen, China; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China.
  • Yang P; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Zhao Y; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Zhuang Z; BGI-Shenzhen, 518103 Shenzhen, China; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China; School of Biology and Biological Engineering, South China University of Technology, 510006 Guangzhou, China.
  • Wang Z; BGI-Shenzhen, 518103 Shenzhen, China.
  • Song R; Center of Infectious Disease, Beijing Ditan Hospital, Capital Medical University, 100015 Beijing, China.
  • Zhang J; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Liu C; BGI-Shenzhen, 518103 Shenzhen, China.
  • Gao Q; BGI-Shenzhen, 518103 Shenzhen, China.
  • Xu Q; BGI-Shenzhen, 518103 Shenzhen, China.
  • Wei X; BGI-Shenzhen, 518103 Shenzhen, China; BGI Education Center, University of Chinese Academy of Sciences, 518083 Shenzhen, China.
  • Sun HX; BGI-Shenzhen, 518103 Shenzhen, China; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China.
  • Ye B; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Wu Y; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Zhang N; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China.
  • Lei G; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Yu L; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Yan J; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Diao G; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Meng F; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Bai C; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Mao P; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Yu Y; BGI-Shenzhen, 518103 Shenzhen, China.
  • Wang M; BGI-Shenzhen, 518103 Shenzhen, China.
  • Yuan Y; BGI-Shenzhen, 518103 Shenzhen, China; BGI Education Center, University of Chinese Academy of Sciences, 518083 Shenzhen, China.
  • Deng Q; BGI-Shenzhen, 518103 Shenzhen, China; BGI Education Center, University of Chinese Academy of Sciences, 518083 Shenzhen, China.
  • Li Z; BGI-Shenzhen, 518103 Shenzhen, China; Northwest A&F University, Yangling, 712100 Shanxi, China.
  • Huang Y; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China.
  • Hu G; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China.
  • Liu Y; BGI-Shenzhen, 518103 Shenzhen, China; BGI Education Center, University of Chinese Academy of Sciences, 518083 Shenzhen, China.
  • Wang X; BGI-Shenzhen, 518103 Shenzhen, China; BGI-GenoImmune, BGI-Shenzhen, 4300794 Wuhan, China.
  • Xu Z; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Liu P; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Bi Y; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China.
  • Shi Y; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China.
  • Zhang S; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China.
  • Chen Z; Center of Infectious Disease, Beijing Ditan Hospital, Capital Medical University, 100015 Beijing, China.
  • Wang J; BGI-Shenzhen, 518103 Shenzhen, China; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China.
  • Xu X; BGI-Shenzhen, 518103 Shenzhen, China; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China.
  • Wu G; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China.
  • Wang FS; Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, 100039 Beijing, China. Electronic address: fswang302@163.com.
  • Gao GF; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing
  • Liu L; BGI-Shenzhen, 518103 Shenzhen, China; China National GeneBank, BGI-Shenzhen, 518120 Shenzhen, China. Electronic address: liulongqi@genomics.cn.
  • Liu WJ; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, China. Electronic address: liujun@ivdc.chinacdc.cn.
Immunity ; 53(3): 685-696.e3, 2020 09 15.
Article in English | MEDLINE | ID: covidwho-716745
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic poses a current world-wide public health threat. However, little is known about its hallmarks compared to other infectious diseases. Here, we report the single-cell transcriptional landscape of longitudinally collected peripheral blood mononuclear cells (PBMCs) in both COVID-19- and influenza A virus (IAV)-infected patients. We observed increase of plasma cells in both COVID-19 and IAV patients and XIAP associated factor 1 (XAF1)-, tumor necrosis factor (TNF)-, and FAS-induced T cell apoptosis in COVID-19 patients. Further analyses revealed distinct signaling pathways activated in COVID-19 (STAT1 and IRF3) versus IAV (STAT3 and NFκB) patients and substantial differences in the expression of key factors. These factors include relatively increase of interleukin (IL)6R and IL6ST expression in COVID-19 patients but similarly increased IL-6 concentrations compared to IAV patients, supporting the clinical observations of increased proinflammatory cytokines in COVID-19 patients. Thus, we provide the landscape of PBMCs and unveil distinct immune response pathways in COVID-19 and IAV patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Leukocytes, Mononuclear / Signal Transduction / Cytokines / Coronavirus Infections / Influenza, Human Type of study: Observational study / Prognostic study Limits: Humans Language: English Journal: Immunity Journal subject: Allergy and Immunology Year: 2020 Document Type: Article Affiliation country: J.immuni.2020.07.009

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Leukocytes, Mononuclear / Signal Transduction / Cytokines / Coronavirus Infections / Influenza, Human Type of study: Observational study / Prognostic study Limits: Humans Language: English Journal: Immunity Journal subject: Allergy and Immunology Year: 2020 Document Type: Article Affiliation country: J.immuni.2020.07.009