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A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2.
Hassan, Ahmed O; Kafai, Natasha M; Dmitriev, Igor P; Fox, Julie M; Smith, Brittany K; Harvey, Ian B; Chen, Rita E; Winkler, Emma S; Wessel, Alex W; Case, James Brett; Kashentseva, Elena; McCune, Broc T; Bailey, Adam L; Zhao, Haiyan; VanBlargan, Laura A; Dai, Ya-Nan; Ma, Meisheng; Adams, Lucas J; Shrihari, Swathi; Danis, Jonathan E; Gralinski, Lisa E; Hou, Yixuan J; Schäfer, Alexandra; Kim, Arthur S; Keeler, Shamus P; Weiskopf, Daniela; Baric, Ralph S; Holtzman, Michael J; Fremont, Daved H; Curiel, David T; Diamond, Michael S.
  • Hassan AO; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Kafai NM; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Dmitriev IP; Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Fox JM; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Smith BK; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Harvey IB; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Chen RE; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Winkler ES; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Wessel AW; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Case JB; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Kashentseva E; Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • McCune BT; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Bailey AL; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Zhao H; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • VanBlargan LA; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Dai YN; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Ma M; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Adams LJ; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Shrihari S; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Danis JE; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Gralinski LE; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
  • Hou YJ; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
  • Schäfer A; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
  • Kim AS; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Keeler SP; Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Weiskopf D; Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.
  • Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
  • Holtzman MJ; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Fremont DH; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Biochemistry and Molecular Biophysics, Washington University School of
  • Curiel DT; Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: dcuriel@wustl.edu.
  • Diamond MS; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 6311
Cell ; 183(1): 169-184.e13, 2020 10 01.
Article in English | MEDLINE | ID: covidwho-720448
ABSTRACT
The coronavirus disease 2019 pandemic has made deployment of an effective vaccine a global health priority. We evaluated the protective activity of a chimpanzee adenovirus-vectored vaccine encoding a prefusion stabilized spike protein (ChAd-SARS-CoV-2-S) in challenge studies with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and mice expressing the human angiotensin-converting enzyme 2 receptor. Intramuscular dosing of ChAd-SARS-CoV-2-S induces robust systemic humoral and cell-mediated immune responses and protects against lung infection, inflammation, and pathology but does not confer sterilizing immunity, as evidenced by detection of viral RNA and induction of anti-nucleoprotein antibodies after SARS-CoV-2 challenge. In contrast, a single intranasal dose of ChAd-SARS-CoV-2-S induces high levels of neutralizing antibodies, promotes systemic and mucosal immunoglobulin A (IgA) and T cell responses, and almost entirely prevents SARS-CoV-2 infection in both the upper and lower respiratory tracts. Intranasal administration of ChAd-SARS-CoV-2-S is a candidate for preventing SARS-CoV-2 infection and transmission and curtailing pandemic spread.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Viral Vaccines / Coronavirus Infections / Immunogenicity, Vaccine Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Female / Humans Language: English Journal: Cell Year: 2020 Document Type: Article Affiliation country: J.cell.2020.08.026

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Viral Vaccines / Coronavirus Infections / Immunogenicity, Vaccine Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Female / Humans Language: English Journal: Cell Year: 2020 Document Type: Article Affiliation country: J.cell.2020.08.026