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IL-6 trans-signaling induces plasminogen activator inhibitor-1 from vascular endothelial cells in cytokine release syndrome.
Kang, Sujin; Tanaka, Toshio; Inoue, Hitomi; Ono, Chikako; Hashimoto, Shoji; Kioi, Yoshiyuki; Matsumoto, Hisatake; Matsuura, Hiroshi; Matsubara, Tsunehiro; Shimizu, Kentaro; Ogura, Hiroshi; Matsuura, Yoshiharu; Kishimoto, Tadamitsu.
  • Kang S; Department of Immune Regulation, Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
  • Tanaka T; Medical Affairs Bureau, Osaka Habikino Medical Center, Osaka 583-8588, Japan.
  • Inoue H; Department of Immune Regulation, Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
  • Ono C; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
  • Hashimoto S; Department of Clinical Laboratory, Osaka Habikino Medical Center, Osaka 583-8588, Japan.
  • Kioi Y; Department of Immune Regulation, Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
  • Matsumoto H; Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
  • Matsuura H; Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
  • Matsubara T; Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
  • Shimizu K; Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
  • Ogura H; Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
  • Matsuura Y; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
  • Kishimoto T; Department of Immune Regulation, Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan; kishimoto@ifrec.osaka-u.ac.jp.
Proc Natl Acad Sci U S A ; 117(36): 22351-22356, 2020 09 08.
Article in English | MEDLINE | ID: covidwho-724752
ABSTRACT
Cytokine release syndrome (CRS) is a life-threatening complication induced by systemic inflammatory responses to infections, including bacteria and chimeric antigen receptor T cell therapy. There are currently no immunotherapies with proven clinical efficacy and understanding of the molecular mechanisms of CRS pathogenesis is limited. Here, we found that patients diagnosed with CRS from sepsis, acute respiratory distress syndrome (ARDS), or burns showed common manifestations strikingly elevated levels of the four proinflammatory cytokines interleukin (IL)-6, IL-8, monocyte chemotactic protein-1 (MCP-1), and IL-10 and the coagulation cascade activator plasminogen activator inhibitor-1 (PAI-1). Our in vitro data indicate that endothelial IL-6 trans-signaling formed an inflammation circuit for robust IL-6, IL-8, and MCP-1 production and promoted PAI-1 production; additionally, an IL-6 signaling blockade by the human monoclonal antibody tocilizumab blunted endothelial cell activation. Plasma from severe COVID-19 patients similarly exhibited increased IL-6, IL-10, and MCP-1 levels, but these levels were not as high as those in patients with CRS from other causes. In contrast, the PAI-1 levels in COVID-19 patients were as highly elevated as those in patients with bacterial sepsis or ARDS. Tocilizumab treatment decreased the PAI-1 levels and alleviated critical illness in severe COVID-19 patients. Our findings suggest that distinct levels of cytokine production are associated with CRS induced by bacterial infection and COVID-19, but both CRS types are accompanied by endotheliopathy through IL-6 trans-signaling. Thus, the present study highlights the crucial role of IL-6 signaling in endothelial dysfunction during bacterial infection and COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Signal Transduction / Interleukin-6 / Plasminogen Activator Inhibitor 1 / Endothelial Cells / Cytokine Release Syndrome Type of study: Prognostic study Language: English Journal: Proc Natl Acad Sci U S A Year: 2020 Document Type: Article Affiliation country: Pnas.2010229117

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Signal Transduction / Interleukin-6 / Plasminogen Activator Inhibitor 1 / Endothelial Cells / Cytokine Release Syndrome Type of study: Prognostic study Language: English Journal: Proc Natl Acad Sci U S A Year: 2020 Document Type: Article Affiliation country: Pnas.2010229117