Proximity Labeling for the Identification of Coronavirus-Host Protein Interactions.
Methods Mol Biol
; 2203: 187-204, 2020.
Article
in English
| MEDLINE | ID: covidwho-729907
ABSTRACT
Biotin-based proximity labeling circumvents major pitfalls of classical biochemical approaches to identify protein-protein interactions. It consists of enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronavirus replicase/transcriptase complex (RTC), i.e., proteins located within a close perimeter of the RTC, can be determined by different proximity labeling approaches using BirAR118G (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viral Proteins
/
Coronavirus
/
Proteomics
/
Enzymes
/
Host-Pathogen Interactions
Limits:
Animals
Language:
English
Journal:
Methods Mol Biol
Journal subject:
Molecular Biology
Year:
2020
Document Type:
Article
Affiliation country:
978-1-0716-0900-2_14
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