Ribopuromycylation in Coronavirus-Infected Cells.
Methods Mol Biol
; 2203: 231-238, 2020.
Article
in English
| MEDLINE | ID: covidwho-729910
ABSTRACT
Ribopuromycylation enables the visualization and quantitation of translation on a cellular level by immunofluorescence or in total using standard western blotting. This technique uses ribosome catalyzed puromycylation of nascent chains followed by immobilization on the ribosome by antibiotic chain elongation inhibitor emetine. Detection of puromycylated ribosome-bound nascent chains can then be achieved using a puromycin-specific antibody.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Puromycin
/
Coronavirus
Limits:
Humans
Language:
English
Journal:
Methods Mol Biol
Journal subject:
Molecular Biology
Year:
2020
Document Type:
Article
Affiliation country:
978-1-0716-0900-2_17
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