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Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19.
Hou, Tieying; Zeng, Weiqi; Yang, Minling; Chen, Wenjing; Ren, Lili; Ai, Jingwen; Wu, Ji; Liao, Yalong; Gou, Xuejing; Li, Yongjun; Wang, Xiaorui; Su, Hang; Gu, Bing; Wang, Jianwei; Xu, Teng.
  • Hou T; Laboratory Medicine, Provincial People's Hospital, Guangdong Academy of Medical Sciences Guangzhou, Guangdong, China.
  • Zeng W; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Yang M; Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province and College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
  • Chen W; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Ren L; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Ai J; NHC Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory, IPB, CAMS-Fondation Mérieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China.
  • Wu J; Department of Infectious Diseases, Huashan Hospital affiliated to Fudan University, Shanghai, China.
  • Liao Y; Laboratory Medicine, Provincial People's Hospital, Guangdong Academy of Medical Sciences Guangzhou, Guangdong, China.
  • Gou X; Laboratory Medicine, Provincial People's Hospital, Guangdong Academy of Medical Sciences Guangzhou, Guangdong, China.
  • Li Y; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Wang X; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Su H; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Gu B; Vision Medicals Center for Infectious Diseases, Guangdong, China.
  • Wang J; Medical Technology School of Xuzhou Medical University, Xuzhou, China.
  • Xu T; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
PLoS Pathog ; 16(8): e1008705, 2020 08.
Article in English | MEDLINE | ID: covidwho-732988
ABSTRACT
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / CRISPR-Cas Systems / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: PLoS Pathog Year: 2020 Document Type: Article Affiliation country: Journal.ppat.1008705

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / CRISPR-Cas Systems / Betacoronavirus Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: PLoS Pathog Year: 2020 Document Type: Article Affiliation country: Journal.ppat.1008705