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Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing.
Farfan, Mauricio J; Torres, Juan P; O'Ryan, Miguel; Olivares, Mauricio; Gallardo, Pablo; Lastra, Jorge; Salas, Carolina.
  • Farfan MJ; Hospital Dr. Luis Calvo Mackenna, Santiago, Chile.
  • Torres JP; Departamento de Pediatría y Cirugía Infantil Oriente, Hospital Dr. Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
  • O'Ryan M; Instituto de Ciencias Biomédicas, e Instituto Milenio de Inmunidad e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
  • Olivares M; Departamento de Pediatría y Cirugía Infantil Oriente, Hospital Dr. Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
  • Gallardo P; Departamento de Pediatría y Cirugía Infantil Oriente, Hospital Dr. Luis Calvo Mackenna, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
  • Lastra J; Hospital Dr. Luis Calvo Mackenna, Santiago, Chile.
  • Salas C; Hospital Dr. Luis Calvo Mackenna, Santiago, Chile.
Rev Chilena Infectol ; 37(3): 276-280, 2020 Jun.
Article in Spanish, English | MEDLINE | ID: covidwho-887641
Preprint
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ABSTRACT
The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In this study, we found that pooling of 5 samples showed that CT variations were in the range of 1.0-4,5 units, with less likelihood of a false negative result. Results of the sample without nucleic acid ex-traction, was unsatisfactory, with a significant increase in CT values, and thus for risk of a false negative result. In conclusion, pooling nasopharyngeal samples with both automated and manual extraction proved reliable, and thus a potential efficient alternative for the diagnosis of suspected COVID-19 in developing countries.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Pandemics / Betacoronavirus Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English / Spanish Journal: Rev Chilena Infectol Year: 2020 Document Type: Article Affiliation country: S0716-10182020000300276

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Pandemics / Betacoronavirus Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English / Spanish Journal: Rev Chilena Infectol Year: 2020 Document Type: Article Affiliation country: S0716-10182020000300276