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Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization.
Azad, Taha; Singaravelu, Ragunath; Crupi, Mathieu J F; Jamieson, Taylor; Dave, Jaahnavi; Brown, Emily E F; Rezaei, Reza; Taha, Zaid; Boulton, Stephen; Martin, Nikolas T; Surendran, Abera; Poutou, Joanna; Ghahremani, Mina; Nouri, Kazem; Whelan, Jack T; Duong, Jessie; Tucker, Sarah; Diallo, Jean-Simon; Bell, John C; Ilkow, Carolina S.
  • Azad T; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Singaravelu R; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Crupi MJF; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Jamieson T; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Dave J; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Brown EEF; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Rezaei R; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Taha Z; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Boulton S; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Martin NT; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Surendran A; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Poutou J; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Ghahremani M; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Nouri K; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Whelan JT; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Duong J; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Tucker S; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Diallo JS; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
  • Bell JC; Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
  • Ilkow CS; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
Membranes (Basel) ; 10(9)2020 Aug 30.
Article in English | MEDLINE | ID: covidwho-736706
ABSTRACT
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic presents an urgent need for an effective vaccine. Molecular characterization of SARS-CoV-2 is critical to the development of effective vaccine and therapeutic strategies. In the present study, we show that the fusion of the SARS-CoV-2 spike protein receptor-binding domain to its transmembrane domain is sufficient to mediate trimerization. Our findings may have implications for vaccine development and therapeutic drug design strategies targeting spike trimerization. As global efforts for developing SARS-CoV-2 vaccines are rapidly underway, we believe this observation is an important consideration for identifying crucial epitopes of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Observational study / Prognostic study Topics: Vaccines Language: English Year: 2020 Document Type: Article Affiliation country: Membranes10090215

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Observational study / Prognostic study Topics: Vaccines Language: English Year: 2020 Document Type: Article Affiliation country: Membranes10090215