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Ultrasensitive and Label-Free Detection of the Measles Virus Using an N-Heterocyclic Carbene-Based Electrochemical Biosensor.
Mayall, Robert M; Smith, Christene A; Hyla, Alexander S; Lee, Dianne S; Crudden, Cathleen M; Birss, Viola I.
  • Mayall RM; Department of Chemistry, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
  • Smith CA; Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada.
  • Hyla AS; Department of Chemistry, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
  • Lee DS; Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada.
  • Crudden CM; Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada.
  • Birss VI; Department of Chemistry, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
ACS Sens ; 5(9): 2747-2752, 2020 09 25.
Article in English | MEDLINE | ID: covidwho-740029
Semantic information from SemMedBD (by NLM)
1. Detection USES Biosensors
Subject
Detection
Predicate
USES
Object
Biosensors
2. Detection CAUSES Infection
Subject
Detection
Predicate
CAUSES
Object
Infection
3. Infection PROCESS_OF Primates
Subject
Infection
Predicate
PROCESS_OF
Object
Primates
4. carbene compared_with Sulfhydryl Compounds
Subject
carbene
Predicate
compared_with
Object
Sulfhydryl Compounds
5. carbene higher_than Sulfhydryl Compounds
Subject
carbene
Predicate
higher_than
Object
Sulfhydryl Compounds
6. Detection USES Biosensors
Subject
Detection
Predicate
USES
Object
Biosensors
7. Detection CAUSES Infection
Subject
Detection
Predicate
CAUSES
Object
Infection
8. Infection PROCESS_OF Primates
Subject
Infection
Predicate
PROCESS_OF
Object
Primates
9. carbene compared_with Sulfhydryl Compounds
Subject
carbene
Predicate
compared_with
Object
Sulfhydryl Compounds
10. carbene higher_than Sulfhydryl Compounds
Subject
carbene
Predicate
higher_than
Object
Sulfhydryl Compounds
ABSTRACT
With the current intense need for rapid and accurate detection of viruses due to COVID-19, we report on a platform technology that is well suited for this purpose, using intact measles virus for a demonstration. Cases of infection due to the measles virus are rapidly increasing, yet current diagnostic tools used to monitor for the virus rely on slow (>1 h) technologies. Here, we demonstrate the first biosensor capable of detecting the measles virus in minutes with no preprocessing steps. The key sensing element is an electrode coated with a self-assembled monolayer containing the measles antibody, immobilized through an N-heterocyclic carbene (NHC). The intact virus is detected by changes in resistance, giving a linear response to 10-100 µg/mL of the intact measles virus without the need to label or process the sample. The limit of detection is 6 µg/mL, which is at the lower limit of concentrations that can cause infections in primates. The NHC-based biosensors are shown to be superior to thiol-based systems, producing an approximately 10× larger response and significantly greater stability toward repeated measurements and long-term storage. This NHC-based biosensor thus represents an important development for both the rapid detection of the measles virus and as a platform technology for the detection of other biological targets of interest.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Benzimidazoles / Biosensing Techniques / Antibodies, Immobilized / Electrochemical Techniques / Measles virus Language: English Journal: ACS Sens Year: 2020 Document Type: Article Affiliation country: Acssensors.0c01250

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Benzimidazoles / Biosensing Techniques / Antibodies, Immobilized / Electrochemical Techniques / Measles virus Language: English Journal: ACS Sens Year: 2020 Document Type: Article Affiliation country: Acssensors.0c01250