Ultrasensitive and Label-Free Detection of the Measles Virus Using an N-Heterocyclic Carbene-Based Electrochemical Biosensor.
ACS Sens
; 5(9): 2747-2752, 2020 09 25.
Article
in English
| MEDLINE | ID: covidwho-740029
ABSTRACT
With the current intense need for rapid and accurate detection of viruses due to COVID-19, we report on a platform technology that is well suited for this purpose, using intact measles virus for a demonstration. Cases of infection due to the measles virus are rapidly increasing, yet current diagnostic tools used to monitor for the virus rely on slow (>1 h) technologies. Here, we demonstrate the first biosensor capable of detecting the measles virus in minutes with no preprocessing steps. The key sensing element is an electrode coated with a self-assembled monolayer containing the measles antibody, immobilized through an N-heterocyclic carbene (NHC). The intact virus is detected by changes in resistance, giving a linear response to 10-100 µg/mL of the intact measles virus without the need to label or process the sample. The limit of detection is 6 µg/mL, which is at the lower limit of concentrations that can cause infections in primates. The NHC-based biosensors are shown to be superior to thiol-based systems, producing an approximately 10× larger response and significantly greater stability toward repeated measurements and long-term storage. This NHC-based biosensor thus represents an important development for both the rapid detection of the measles virus and as a platform technology for the detection of other biological targets of interest.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Benzimidazoles
/
Biosensing Techniques
/
Antibodies, Immobilized
/
Electrochemical Techniques
/
Measles virus
Language:
English
Journal:
ACS Sens
Year:
2020
Document Type:
Article
Affiliation country:
Acssensors.0c01250
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