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Human B Cell Clonal Expansion and Convergent Antibody Responses to SARS-CoV-2.
Nielsen, Sandra C A; Yang, Fan; Jackson, Katherine J L; Hoh, Ramona A; Röltgen, Katharina; Jean, Grace H; Stevens, Bryan A; Lee, Ji-Yeun; Rustagi, Arjun; Rogers, Angela J; Powell, Abigail E; Hunter, Molly; Najeeb, Javaria; Otrelo-Cardoso, Ana R; Yost, Kathryn E; Daniel, Bence; Nadeau, Kari C; Chang, Howard Y; Satpathy, Ansuman T; Jardetzky, Theodore S; Kim, Peter S; Wang, Taia T; Pinsky, Benjamin A; Blish, Catherine A; Boyd, Scott D.
  • Nielsen SCA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Yang F; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Jackson KJL; Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.
  • Hoh RA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Röltgen K; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Jean GH; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
  • Stevens BA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Lee JY; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Rustagi A; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA.
  • Rogers AJ; Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University, Stanford, CA 94305, USA.
  • Powell AE; Stanford ChEM-H and Department of Biochemistry, Stanford University, Stanford, CA 94305, USA.
  • Hunter M; ATUM, Newark, CA, 94560, USA.
  • Najeeb J; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA.
  • Otrelo-Cardoso AR; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA.
  • Yost KE; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Daniel B; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Nadeau KC; Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University, Stanford, CA 94305, USA; Sean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Chang HY; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
  • Satpathy AT; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Jardetzky TS; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA; Sean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, Stanford, CA, 94305, USA.
  • Kim PS; Stanford ChEM-H and Department of Biochemistry, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.
  • Wang TT; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.
  • Pinsky BA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
  • Blish CA; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: cblish@stanford.edu.
  • Boyd SD; Department of Pathology, Stanford University, Stanford, CA 94305, USA; Sean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, Stanford, CA, 94305, USA. Electronic address: sboyd1@stanford.edu.
Cell Host Microbe ; 28(4): 516-525.e5, 2020 10 07.
Article in English | MEDLINE | ID: covidwho-743914
ABSTRACT
B cells are critical for the production of antibodies and protective immunity to viruses. Here we show that patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) who develop coronavirus disease 2019 (COVID-19) display early recruitment of B cells expressing a limited subset of IGHV genes, progressing to a highly polyclonal response of B cells with broader IGHV gene usage and extensive class switching to IgG and IgA subclasses with limited somatic hypermutation in the initial weeks of infection. We identify convergence of antibody sequences across SARS-CoV-2-infected patients, highlighting stereotyped naive responses to this virus. Notably, sequence-based detection in COVID-19 patients of convergent B cell clonotypes previously reported in SARS-CoV infection predicts the presence of SARS-CoV/SARS-CoV-2 cross-reactive antibody titers specific for the receptor-binding domain. These findings offer molecular insights into shared features of human B cell responses to SARS-CoV-2 and SARS-CoV.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / B-Lymphocytes / Coronavirus Infections / Betacoronavirus / Antibodies, Viral Type of study: Prognostic study / Randomized controlled trials Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Cell Host Microbe Journal subject: Microbiology Year: 2020 Document Type: Article Affiliation country: J.chom.2020.09.002

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / B-Lymphocytes / Coronavirus Infections / Betacoronavirus / Antibodies, Viral Type of study: Prognostic study / Randomized controlled trials Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Cell Host Microbe Journal subject: Microbiology Year: 2020 Document Type: Article Affiliation country: J.chom.2020.09.002