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Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19.
Peng, Yanchun; Mentzer, Alexander J; Liu, Guihai; Yao, Xuan; Yin, Zixi; Dong, Danning; Dejnirattisai, Wanwisa; Rostron, Timothy; Supasa, Piyada; Liu, Chang; López-Camacho, César; Slon-Campos, Jose; Zhao, Yuguang; Stuart, David I; Paesen, Guido C; Grimes, Jonathan M; Antson, Alfred A; Bayfield, Oliver W; Hawkins, Dorothy E D P; Ker, De-Sheng; Wang, Beibei; Turtle, Lance; Subramaniam, Krishanthi; Thomson, Paul; Zhang, Ping; Dold, Christina; Ratcliff, Jeremy; Simmonds, Peter; de Silva, Thushan; Sopp, Paul; Wellington, Dannielle; Rajapaksa, Ushani; Chen, Yi-Ling; Salio, Mariolina; Napolitani, Giorgio; Paes, Wayne; Borrow, Persephone; Kessler, Benedikt M; Fry, Jeremy W; Schwabe, Nikolai F; Semple, Malcolm G; Baillie, J Kenneth; Moore, Shona C; Openshaw, Peter J M; Ansari, M Azim; Dunachie, Susanna; Barnes, Eleanor; Frater, John; Kerr, Georgina; Goulder, Philip.
  • Peng Y; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Mentzer AJ; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Liu G; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Yao X; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Yin Z; Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
  • Dong D; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Dejnirattisai W; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Rostron T; Beijing You'an Hospital, Capital Medical University, Beijing, China.
  • Supasa P; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Liu C; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • López-Camacho C; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Slon-Campos J; MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Zhao Y; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Stuart DI; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Paesen GC; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Grimes JM; CAMS Key Laboratory of Tumor Immunology and Radiation Therapy, Xinjiang Tumor Hospital, Xinjiang Medical University, Xinjiang, China.
  • Antson AA; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Bayfield OW; Sequencing and Flow Cytometry Facility, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
  • Hawkins DEDP; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Ker DS; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Wang B; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Turtle L; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Subramaniam K; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Thomson P; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Zhang P; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Dold C; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Ratcliff J; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Simmonds P; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • de Silva T; Diamond Light Source, Didcot, UK.
  • Sopp P; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Wellington D; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Rajapaksa U; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Chen YL; Diamond Light Source, Didcot, UK.
  • Salio M; York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.
  • Napolitani G; York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.
  • Paes W; York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.
  • Borrow P; York Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.
  • Kessler BM; Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), University of Oxford, Oxford, UK.
  • Fry JW; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Schwabe NF; Tropical and Infectious Diseases Unit, Liverpool University Hospitals NHS Foundation Trust, Liverpool, UK.
  • Semple MG; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, UK.
  • Baillie JK; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, UK.
  • Moore SC; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, UK.
  • Openshaw PJM; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Ansari MA; Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, UK.
  • Dunachie S; NIHR Oxford Biomedical Research Centre, Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, Oxford, UK.
  • Barnes E; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Frater J; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • Kerr G; The Florey Institute for Host-Pathogen Interactions, Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield, UK.
  • Goulder P; Sequencing and Flow Cytometry Facility, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Nat Immunol ; 21(11): 1336-1345, 2020 11.
Article in English | MEDLINE | ID: covidwho-889210
ABSTRACT
The development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and therapeutics will depend on understanding viral immunity. We studied T cell memory in 42 patients following recovery from COVID-19 (28 with mild disease and 14 with severe disease) and 16 unexposed donors, using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses were significantly higher in severe as compared with mild cases. Total and spike-specific T cell responses correlated with spike-specific antibody responses. We identified 41 peptides containing CD4+ and/or CD8+ epitopes, including six immunodominant regions. Six optimized CD8+ epitopes were defined, with peptide-MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild cases, higher proportions of SARS-CoV-2-specific CD8+ T cells were observed. The identification of T cell responses associated with milder disease will support an understanding of protective immunity and highlights the potential of including non-spike proteins within future COVID-19 vaccine design.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: CD4-Positive T-Lymphocytes / CD8-Positive T-Lymphocytes / Betacoronavirus / Immunologic Memory / Antigens, Viral Type of study: Observational study Topics: Vaccines Limits: Humans Country/Region as subject: Europa Language: English Journal: Nat Immunol Journal subject: Allergy and Immunology Year: 2020 Document Type: Article Affiliation country: S41590-020-0782-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: CD4-Positive T-Lymphocytes / CD8-Positive T-Lymphocytes / Betacoronavirus / Immunologic Memory / Antigens, Viral Type of study: Observational study Topics: Vaccines Limits: Humans Country/Region as subject: Europa Language: English Journal: Nat Immunol Journal subject: Allergy and Immunology Year: 2020 Document Type: Article Affiliation country: S41590-020-0782-6