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Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset.
Lou, Bin; Li, Ting-Dong; Zheng, Shu-Fa; Su, Ying-Ying; Li, Zhi-Yong; Liu, Wei; Yu, Fei; Ge, Sheng-Xiang; Zou, Qian-Da; Yuan, Quan; Lin, Sha; Hong, Cong-Ming; Yao, Xiang-Yang; Zhang, Xue-Jie; Wu, Ding-Hui; Zhou, Guo-Liang; Hou, Wang-Heng; Li, Ting-Ting; Zhang, Ya-Li; Zhang, Shi-Yin; Fan, Jian; Zhang, Jun; Xia, Ning-Shao; Chen, Yu.
  • Lou B; Dept of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
  • Li TD; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
  • Zheng SF; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, China.
  • Su YY; Bin Lou, Ting-Dong Li, Shu-Fa Zheng and Ying-Ying Su contributed equally to this article.
  • Li ZY; The State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, School of Public Health and School of Life Science, Xiamen University, Xiamen, China.
  • Liu W; School of Public Health, Xiamen University, Xiamen, China.
  • Yu F; Bin Lou, Ting-Dong Li, Shu-Fa Zheng and Ying-Ying Su contributed equally to this article.
  • Ge SX; Dept of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
  • Zou QD; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
  • Yuan Q; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, China.
  • Lin S; Bin Lou, Ting-Dong Li, Shu-Fa Zheng and Ying-Ying Su contributed equally to this article.
  • Hong CM; The State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, School of Public Health and School of Life Science, Xiamen University, Xiamen, China.
  • Yao XY; School of Public Health, Xiamen University, Xiamen, China.
  • Zhang XJ; Bin Lou, Ting-Dong Li, Shu-Fa Zheng and Ying-Ying Su contributed equally to this article.
  • Wu DH; School of Public Health, Xiamen University, Xiamen, China.
  • Zhou GL; The State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, School of Public Health and School of Life Science, Xiamen University, Xiamen, China.
  • Hou WH; School of Public Health, Xiamen University, Xiamen, China.
  • Li TT; Dept of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
  • Zhang YL; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
  • Zhang SY; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, China.
  • Fan J; The State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, School of Public Health and School of Life Science, Xiamen University, Xiamen, China.
  • Zhang J; School of Public Health, Xiamen University, Xiamen, China.
  • Xia NS; Yu Chen, Jian Fan, Sheng-Xiang Ge and Jun Zhang contributed equally to this article as lead authors and jointly supervised the work.
  • Chen Y; Dept of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Eur Respir J ; 56(2)2020 08.
Article in English | MEDLINE | ID: covidwho-744959
Semantic information from SemMedBD (by NLM)
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ABSTRACT

BACKGROUND:

Timely diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is a prerequisite for treatment and prevention. The serology characteristics and complement diagnosis value of the antibody test to RNA test need to be demonstrated.

METHOD:

Serial sera of 80 patients with PCR-confirmed coronavirus disease 2019 (COVID-19) were collected at the First Affiliated Hospital of Zhejiang University, Hangzhou, China. Total antibody (Ab), IgM and IgG antibodies against SARS-CoV-2 were detected, and the antibody dynamics during the infection were described.

RESULTS:

The seroconversion rates for Ab, IgM and IgG were 98.8%, 93.8% and 93.8%, respectively. The first detectible serology marker was Ab, followed by IgM and IgG, with a median seroconversion time of 15, 18 and 20 days post exposure (d.p.e.) or 9, 10 and 12 days post onset (d.p.o.), respectively. The antibody levels increased rapidly beginning at 6 d.p.o. and were accompanied by a decline in viral load. For patients in the early stage of illness (0-7 d.p.o), Ab showed the highest sensitivity (64.1%) compared with IgM and IgG (33.3% for both; p<0.001). The sensitivities of Ab, IgM and IgG increased to 100%, 96.7% and 93.3%, respectively, 2 weeks later. When the same antibody type was detected, no significant difference was observed between enzyme-linked immunosorbent assays and other forms of immunoassays.

CONCLUSIONS:

A typical acute antibody response is induced during SARS-CoV-2 infection. Serology testing provides an important complement to RNA testing in the later stages of illness for pathogenic-specific diagnosis and helpful information to evaluate the adapted immunity status of patients.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Type of study: Diagnostic study Language: English Clinical aspect: Diagnosis Year: 2020

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Subject: Pneumonia, Viral / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus Type of study: Diagnostic study Language: English Clinical aspect: Diagnosis Year: 2020
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