Your browser doesn't support javascript.
Structures of the SARS-CoV-2 nucleocapsid and their perspectives for drug design.
Peng, Ya; Du, Ning; Lei, Yuqing; Dorje, Sonam; Qi, Jianxun; Luo, Tingrong; Gao, George F; Song, Hao.
  • Peng Y; Laboratory of Animal Infectious Diseases, College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning, China.
  • Du N; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • Lei Y; Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.
  • Dorje S; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • Qi J; University of Chinese Academy of Sciences, Beijing, China.
  • Luo T; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • Gao GF; University of Chinese Academy of Sciences, Beijing, China.
  • Song H; CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
EMBO J ; 39(20): e105938, 2020 10 15.
Article in English | MEDLINE | ID: covidwho-750343
Semantic information from SemMedBD (by NLM)
1. Nucleocapsid PART_OF 2019 novel coronavirus
Subject
Nucleocapsid
Predicate
PART_OF
Object
2019 novel coronavirus
2. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
3. Nucleocapsid CAUSES Borg Category-Ratio 10 Perceived Exertion Score 5
Subject
Nucleocapsid
Predicate
CAUSES
Object
Borg Category-Ratio 10 Perceived Exertion Score 5
4. Virion AFFECTS Virus Replication
Subject
Virion
Predicate
AFFECTS
Object
Virus Replication
5. Small Molecule DISRUPTS Virus Diseases
Subject
Small Molecule
Predicate
DISRUPTS
Object
Virus Diseases
6. Small Molecule INTERACTS_WITH Binding Sites
Subject
Small Molecule
Predicate
INTERACTS_WITH
Object
Binding Sites
7. Nucleocapsid PART_OF 2019 novel coronavirus
Subject
Nucleocapsid
Predicate
PART_OF
Object
2019 novel coronavirus
8. 2019 novel coronavirus CAUSES COVID-19
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
COVID-19
9. Nucleocapsid CAUSES Borg Category-Ratio 10 Perceived Exertion Score 5
Subject
Nucleocapsid
Predicate
CAUSES
Object
Borg Category-Ratio 10 Perceived Exertion Score 5
10. Virion AFFECTS Virus Replication
Subject
Virion
Predicate
AFFECTS
Object
Virus Replication
11. Small Molecule DISRUPTS Virus Diseases
Subject
Small Molecule
Predicate
DISRUPTS
Object
Virus Diseases
12. Small Molecule INTERACTS_WITH Binding Sites
Subject
Small Molecule
Predicate
INTERACTS_WITH
Object
Binding Sites
ABSTRACT
COVID-19, caused by SARS-CoV-2, has resulted in severe and unprecedented economic and social disruptions in the world. Nucleocapsid (N) protein, which is the major structural component of the virion and is involved in viral replication, assembly and immune regulation, plays key roles in the viral life cycle. Here, we solved the crystal structures of the N- and C-terminal domains (N-NTD and N-CTD) of SARS-CoV-2 N protein, at 1.8 and 1.5 Å resolution, respectively. Both structures show conserved features from other CoV N proteins. The binding sites targeted by small molecules against HCoV-OC43 and MERS-CoV, which inhibit viral infection by blocking the RNA-binding activity or normal oligomerization of N protein, are relatively conserved in our structure, indicating N protein is a promising drug target. In addition, certain areas of N-NTD and N-CTD display distinct charge distribution patterns in SARS-CoV-2, which may alter the RNA-binding modes. The specific antigenic characteristics are critical for developing specific immune-based rapid diagnostic tests. Our structural information can aid in the discovery and development of antiviral inhibitors against SARS-CoV-2 in the future.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Drug Design / Nucleocapsid Proteins / Betacoronavirus Type of study: Diagnostic study Limits: Humans Language: English Journal: EMBO J Year: 2020 Document Type: Article Affiliation country: Embj.2020105938

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Drug Design / Nucleocapsid Proteins / Betacoronavirus Type of study: Diagnostic study Limits: Humans Language: English Journal: EMBO J Year: 2020 Document Type: Article Affiliation country: Embj.2020105938