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Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone.
Zhao, Hui; Liu, Feng; Xie, Wei; Zhou, Tai-Cheng; OuYang, Jun; Jin, Lian; Li, Hui; Zhao, Chun-Yan; Zhang, Liang; Wei, Jia; Zhang, Ya-Ping; Li, Can-Peng.
  • Zhao H; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
  • Liu F; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
  • Xie W; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
  • Zhou TC; Central Lab, Liver Disease Research Center, the Second People's Hospital of Yunnan Province, Kunming, 650021, China.
  • OuYang J; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
  • Jin L; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
  • Li H; Central Lab, Liver Disease Research Center, the Second People's Hospital of Yunnan Province, Kunming, 650021, China.
  • Zhao CY; Central Lab, Liver Disease Research Center, the Second People's Hospital of Yunnan Province, Kunming, 650021, China.
  • Zhang L; Central Lab, Liver Disease Research Center, the Second People's Hospital of Yunnan Province, Kunming, 650021, China.
  • Wei J; Central Lab, Liver Disease Research Center, the Second People's Hospital of Yunnan Province, Kunming, 650021, China.
  • Zhang YP; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
  • Li CP; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.
Sens Actuators B Chem ; 327: 128899, 2021 Jan 15.
Article in English | MEDLINE | ID: covidwho-756853
ABSTRACT
The recent pandemic outbreak of COVID-19 caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses a threat to public health globally. Thus, developing a rapid, accurate, and easy-to-implement diagnostic system for SARS-CoV-2 is crucial for controlling infection sources and monitoring illness progression. Here, we reported an ultrasensitive electrochemical detection technology using calixarene functionalized graphene oxide for targeting RNA of SARS-CoV-2. Based on a supersandwich-type recognition strategy, the technology was confirmed to practicably detect the RNA of SARS-CoV-2 without nucleic acid amplification and reverse-transcription by using a portable electrochemical smartphone. The biosensor showed high specificity and selectivity during in silico analysis and actual testing. A total of 88 RNA extracts from 25 SARS-CoV-2-confirmed patients and eight recovery patients were detected using the biosensor. The detectable ratios (85.5 % and 46.2 %) were higher than those obtained using RT-qPCR (56.5 % and 7.7 %). The limit of detection (LOD) of the clinical specimen was 200 copies/mL, which is the lowest LOD among the published RNA measurement of SARS-CoV-2 to date. Additionally, only two copies (10 µL) of SARS-CoV-2 were required for per assay. Therefore, we developed an ultrasensitive, accurate, and convenient assay for SARS-CoV-2 detection, providing a potential method for point-of-care testing.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Sens Actuators B Chem Year: 2021 Document Type: Article Affiliation country: J.snb.2020.128899

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Prognostic study Language: English Journal: Sens Actuators B Chem Year: 2021 Document Type: Article Affiliation country: J.snb.2020.128899