Quantification of Coronaviruses by Titration In Vitro and Ex Vivo.
Methods Mol Biol
; 2203: 135-143, 2020.
Article
in English
| MEDLINE | ID: covidwho-761350
ABSTRACT
Several techniques are currently available to quickly and accurately quantify the number of virus particles in a sample, taking advantage of advanced technologies improving old techniques or generating new ones, generally relying on partial detection methods or structural analysis. Therefore, characterization of virus infectivity in a sample is often essential, and classical virological methods are extremely powerful in providing accurate results even in an old-fashioned way. In this chapter, we describe in detail the techniques routinely used to estimate the number of viable infectious coronavirus particles in a given sample. All these techniques are serial dilution assays, also known as titrations or end-point dilution assays (EPDA).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viral Plaque Assay
/
Coronavirus
Limits:
Animals
Language:
English
Journal:
Methods Mol Biol
Journal subject:
Molecular Biology
Year:
2020
Document Type:
Article
Affiliation country:
978-1-0716-0900-2_11
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