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Visualizing Coronavirus Entry into Cells.
Milewska, Aleksandra; Owczarek, Katarzyna; Szczepanski, Artur; Pyrc, Krzysztof.
  • Milewska A; Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
  • Owczarek K; Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
  • Szczepanski A; Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
  • Pyrc K; Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. k.a.pyrc@uj.edu.pl.
Methods Mol Biol ; 2203: 241-261, 2020.
Article in English | MEDLINE | ID: covidwho-729911
ABSTRACT
Coronavirus entry encompasses the initial steps of infection, from virion attachment to genome release. Advances in fluorescent labeling of viral and cellular components and confocal imaging enable broad spectrum studies on this process. Here, we describe methods for visualization of coronavirus entry into immortalized cell lines and 3D tissue culture models.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus / Microscopy, Confocal / Host-Pathogen Interactions Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2020 Document Type: Article Affiliation country: 978-1-0716-0900-2_18

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus / Microscopy, Confocal / Host-Pathogen Interactions Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2020 Document Type: Article Affiliation country: 978-1-0716-0900-2_18