Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018.
Emerg Microbes Infect
; 9(1): 246-255, 2020.
Article
in English
| MEDLINE | ID: covidwho-774889
ABSTRACT
Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507â
L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507â
L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Respiratory Tract Infections
/
Coronavirus Infections
/
Coronavirus NL63, Human
Type of study:
Randomized controlled trials
Limits:
Child, preschool
/
Humans
/
Infant
Country/Region as subject:
Asia
Language:
English
Journal:
Emerg Microbes Infect
Year:
2020
Document Type:
Article
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