Your browser doesn't support javascript.
The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device.
Baker, Alexander N; Richards, Sarah-Jane; Guy, Collette S; Congdon, Thomas R; Hasan, Muhammad; Zwetsloot, Alexander J; Gallo, Angelo; Lewandowski, Józef R; Stansfeld, Phillip J; Straube, Anne; Walker, Marc; Chessa, Simona; Pergolizzi, Giulia; Dedola, Simone; Field, Robert A; Gibson, Matthew I.
  • Baker AN; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Richards SJ; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Guy CS; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Congdon TR; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, U.K.
  • Hasan M; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Zwetsloot AJ; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Gallo A; Warwick Medical School, University of Warwick, Coventry, CV4 7AL, U.K.
  • Lewandowski JR; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Stansfeld PJ; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Straube A; Department of Chemistry, University of Warwick, Coventry, CV4 7AL, U.K.
  • Walker M; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, U.K.
  • Chessa S; Warwick Medical School, University of Warwick, Coventry, CV4 7AL, U.K.
  • Pergolizzi G; Department of Physics, University of Warwick, Coventry, CV4 7AL, U.K.
  • Dedola S; Iceni Diagnostics Ltd, Norwich Research Park, Norwich, NR4 7GJ, U.K.
  • Field RA; Iceni Diagnostics Ltd, Norwich Research Park, Norwich, NR4 7GJ, U.K.
  • Gibson MI; Iceni Diagnostics Ltd, Norwich Research Park, Norwich, NR4 7GJ, U.K.
ACS Cent Sci ; 6(11): 2046-2052, 2020 Nov 25.
Article in English | MEDLINE | ID: covidwho-779921
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: ACS Cent Sci Year: 2020 Document Type: Article Affiliation country: Acscentsci.0c00855

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: ACS Cent Sci Year: 2020 Document Type: Article Affiliation country: Acscentsci.0c00855