Immunopathogenesis of infectious bronchitis virus Q1 in specific pathogen free chicks.
Microb Pathog
; 149: 104535, 2020 Dec.
Article
in English
| MEDLINE | ID: covidwho-792800
ABSTRACT
The immunopathogenesis of avian coronavirus, infectious bronchitis virus (IBV) Q1, was investigated in specific pathogen free chicks. Following infection, chicks exhibited respiratory clinical signs and reduced body weight. Oropharyngeal (OP) and cloacal (CL) swabs were collected at intervals and found to be RT-PCR positive, with a greater number of partial-S1 amino acid changes noted in CL swabs compared to OP swabs. In tissue samples, IBV viral load peaked 9 days post infection (dpi) in the trachea and kidneys, and 14 dpi in the proventriculus. At 28 dpi, ELISA data showed that 63% of infected chicks seroconverted. There was significantly higher mRNA up-regulation of IFN-α, TLR3, MDA5, LITAF, IL-1ß and IL-6 in the trachea compared to the kidneys. Findings presented here demonstrate that this Q1 isolate induces greater lesions and host innate immune responses in chickens' tracheas compared to the kidneys.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Poultry Diseases
/
Chickens
/
Coronavirus Infections
/
Infectious bronchitis virus
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Microb Pathog
Journal subject:
Communicable Diseases
/
Microbiology
Year:
2020
Document Type:
Article
Affiliation country:
J.micpath.2020.104535
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