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ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens.
Zamecnik, Colin R; Rajan, Jayant V; Yamauchi, Kevin A; Mann, Sabrina A; Loudermilk, Rita P; Sowa, Gavin M; Zorn, Kelsey C; Alvarenga, Bonny D; Gaebler, Christian; Caskey, Marina; Stone, Mars; Norris, Philip J; Gu, Wei; Chiu, Charles Y; Ng, Dianna; Byrnes, James R; Zhou, Xin X; Wells, James A; Robbiani, Davide F; Nussenzweig, Michel C; DeRisi, Joseph L; Wilson, Michael R.
  • Zamecnik CR; Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, San Francisco, CA, USA.
  • Rajan JV; Division of Experimental Medicine, Department of Medicine, Zuckerberg San Francisco General Hospital, University of California, San Francisco, San Francisco, CA, USA.
  • Yamauchi KA; Chan Zuckerberg Biohub, San Francisco, CA, USA.
  • Mann SA; Chan Zuckerberg Biohub, San Francisco, CA, USA.
  • Loudermilk RP; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
  • Sowa GM; Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, San Francisco, CA, USA.
  • Zorn KC; University of California, San Francisco School of Medicine, San Francisco, CA, USA.
  • Alvarenga BD; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
  • Gaebler C; Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, San Francisco, CA, USA.
  • Caskey M; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
  • Stone M; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
  • Norris PJ; Vitalant Research Institute, San Francisco, CA, USA.
  • Gu W; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.
  • Chiu CY; Vitalant Research Institute, San Francisco, CA, USA.
  • Ng D; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.
  • Byrnes JR; Department of Medicine, University of California, San Francisco, San Francisco, USA.
  • Zhou XX; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.
  • Wells JA; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.
  • Robbiani DF; Department of Medicine, University of California, San Francisco, San Francisco, USA.
  • Nussenzweig MC; Department of Medicine, University of California, San Francisco, San Francisco, USA.
  • DeRisi JL; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, USA.
  • Wilson MR; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, USA.
Cell Rep Med ; 1(7): 100123, 2020 10 20.
Article in English | MEDLINE | ID: covidwho-793949
ABSTRACT
Comprehensive understanding of the serological response to SARS-CoV-2 infection is important for both pathophysiologic insight and diagnostic development. Here, we generate a pan-human coronavirus programmable phage display assay to perform proteome-wide profiling of coronavirus antigens enriched by 98 COVID-19 patient sera. Next, we use ReScan, a method to efficiently sequester phage expressing the most immunogenic peptides and print them onto paper-based microarrays using acoustic liquid handling, which isolates and identifies nine candidate antigens, eight of which are derived from the two proteins used for SARS-CoV-2 serologic assays spike and nucleocapsid proteins. After deployment in a high-throughput assay amenable to clinical lab settings, these antigens show improved specificity over a whole protein panel. This proof-of-concept study demonstrates that ReScan will have broad applicability for other emerging infectious diseases or autoimmune diseases that lack a valid biomarker, enabling a seamless pipeline from antigen discovery to diagnostic using one recombinant protein source.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antigens, Viral Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Female / Humans / Male / Middle aged Language: English Journal: Cell Rep Med Year: 2020 Document Type: Article Affiliation country: J.xcrm.2020.100123

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 / Antigens, Viral Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Female / Humans / Male / Middle aged Language: English Journal: Cell Rep Med Year: 2020 Document Type: Article Affiliation country: J.xcrm.2020.100123