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Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Platform to Study SARS-CoV-2 Related Myocardial Injury.
Wong, Chun-Ka; Luk, Hayes Kam-Hei; Lai, Wing-Hon; Lau, Yee-Man; Zhang, Ricky Ruiqi; Wong, Antonio Cheuk-Pui; Lo, George Chi-Shing; Chan, Kwok-Hung; Hung, Ivan Fan-Ngai; Tse, Hung-Fat; Woo, Patrick Chiu-Yat; Lau, Susanna Kar-Pui; Siu, Chung-Wah.
  • Wong CK; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Luk HK; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Lai WH; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Lau YM; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Zhang RR; Infectious Disease Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Wong AC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Lo GC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Chan KH; Infectious Disease Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Hung IF; Infectious Disease Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Tse HF; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Woo PC; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Lau SK; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
  • Siu CW; Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
Circ J ; 84(11): 2027-2031, 2020 10 23.
Article in English | MEDLINE | ID: covidwho-795948
ABSTRACT

BACKGROUND:

SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.Methods and 

Results:

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated.

CONCLUSIONS:

Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Myocytes, Cardiac / Induced Pluripotent Stem Cells / Betacoronavirus / Myocarditis Type of study: Prognostic study Topics: Long Covid / Traditional medicine Limits: Humans Language: English Journal: Circ J Journal subject: Vascular Diseases / Cardiology Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Coronavirus Infections / Myocytes, Cardiac / Induced Pluripotent Stem Cells / Betacoronavirus / Myocarditis Type of study: Prognostic study Topics: Long Covid / Traditional medicine Limits: Humans Language: English Journal: Circ J Journal subject: Vascular Diseases / Cardiology Year: 2020 Document Type: Article