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Programmable low-cost DNA-based platform for viral RNA detection.
Zhou, Lifeng; Chandrasekaran, Arun Richard; Punnoose, Jibin Abraham; Bonenfant, Gaston; Charles, Stephon; Levchenko, Oksana; Badu, Pheonah; Cavaliere, Cassandra; Pager, Cara T; Halvorsen, Ken.
  • Zhou L; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Chandrasekaran AR; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Punnoose JA; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Bonenfant G; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Charles S; Department of Biology, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Levchenko O; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Badu P; Department of Biology, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Cavaliere C; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Pager CT; The RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA.
  • Halvorsen K; Department of Biology, University at Albany, State University of New York, Albany, NY 12222, USA.
Sci Adv ; 6(39)2020 09.
Article in English | MEDLINE | ID: covidwho-796906
ABSTRACT
Detection of viruses is critical for controlling disease spread. Recent emerging viral threats, including Zika virus, Ebola virus, and SARS-CoV-2 responsible for coronavirus disease 2019 (COVID-19) highlight the cost and difficulty in responding rapidly. To address these challenges, we develop a platform for low-cost and rapid detection of viral RNA with DNA nanoswitches that mechanically reconfigure in response to specific viruses. Using Zika virus as a model system, we show nonenzymatic detection of viral RNA with selective and multiplexed detection between related viruses and viral strains. For clinical-level sensitivity in biological fluids, we paired the assay with sample preparation using either RNA extraction or isothermal preamplification. Our assay requires minimal laboratory infrastructure and is adaptable to other viruses, as demonstrated by quickly developing DNA nanoswitches to detect SARS-CoV-2 RNA in saliva. Further development and field implementation will improve our ability to detect emergent viral threats and ultimately limit their impact.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / DNA, Single-Stranded / RNA, Viral / Sequence Analysis, RNA / Coronavirus Infections / Electrophoresis, Agar Gel / Betacoronavirus Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2020 Document Type: Article Affiliation country: Sciadv.abc6246

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / DNA, Single-Stranded / RNA, Viral / Sequence Analysis, RNA / Coronavirus Infections / Electrophoresis, Agar Gel / Betacoronavirus Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Year: 2020 Document Type: Article Affiliation country: Sciadv.abc6246