Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay.
Nat Protoc
; 15(11): 3699-3715, 2020 11.
Article
in English
| MEDLINE | ID: covidwho-797534
ABSTRACT
Pseudotyped viruses are useful virological tools because of their safety and versatility. On the basis of a vesicular stomatitis virus (VSV) pseudotyped virus production system, we developed a pseudotyped virus-based neutralization assay against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in biosafety level 2 facilities. Compared with the binding antibody test, the neutralization assay could discriminate the protective agents from the antibody family. This protocol includes production and titration of the SARS-CoV-2 S pseudotyped virus and the neutralization assay based on it. Various types of samples targeting virus attachment and entry could be evaluated for their potency, including serum samples derived from animals and humans, monoclonal antibodies and fusion inhibitors (peptides or small molecules). If the pseudotyped virus stock has been prepared in advance, it will take 2 days to get the potency data for the candidate samples. Experience in handling cells is needed before implementing this protocol.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
Genetic Techniques
/
Coronavirus Infections
/
Antibodies, Neutralizing
/
Betacoronavirus
Type of study:
Experimental Studies
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Nat Protoc
Year:
2020
Document Type:
Article
Affiliation country:
S41596-020-0394-5
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