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SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene.
Ziegler, Katharina; Steininger, Philipp; Ziegler, Renate; Steinmann, Jörg; Korn, Klaus; Ensser, Armin.
  • Ziegler K; Institute of Virology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU) Erlangen, Germany.
  • Steininger P; Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Paracelsus Medical University, Nuremberg, Germany.
  • Ziegler R; Institute of Virology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU) Erlangen, Germany.
  • Steinmann J; Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Paracelsus Medical University, Nuremberg, Germany.
  • Korn K; Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Paracelsus Medical University, Nuremberg, Germany.
  • Ensser A; Institute of Virology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU) Erlangen, Germany.
Euro Surveill ; 25(39)2020 10.
Article in English | MEDLINE | ID: covidwho-808986
ABSTRACT
We found that a single nucleotide polymorphism (SNP) in the nucleoprotein gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a patient interfered with detection in a widely used commercial assay. Some 0.2% of the isolates in the EpiCoV database contain this SNP. Although SARS-CoV-2 was still detected by the other probe in the assay, this underlines the necessity of targeting two independent essential regions of a pathogen for reliable detection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Viral Proteins / RNA, Viral / Point Mutation / Coronavirus Infections / Clinical Laboratory Techniques / Polymorphism, Single Nucleotide / Pandemics / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Case report / Diagnostic study / Prognostic study / Randomized controlled trials Limits: Female / Humans / Middle aged Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.39.2001650

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Viral Proteins / RNA, Viral / Point Mutation / Coronavirus Infections / Clinical Laboratory Techniques / Polymorphism, Single Nucleotide / Pandemics / Real-Time Polymerase Chain Reaction / Betacoronavirus Type of study: Case report / Diagnostic study / Prognostic study / Randomized controlled trials Limits: Female / Humans / Middle aged Country/Region as subject: Europa Language: English Journal subject: Communicable Diseases Year: 2020 Document Type: Article Affiliation country: 1560-7917.ES.2020.25.39.2001650