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Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2).
Runfeng, Li; Yunlong, Hou; Jicheng, Huang; Weiqi, Pan; Qinhai, Ma; Yongxia, Shi; Chufang, Li; Jin, Zhao; Zhenhua, Jia; Haiming, Jiang; Kui, Zheng; Shuxiang, Huang; Jun, Dai; Xiaobo, Li; Xiaotao, Hou; Lin, Wang; Nanshan, Zhong; Zifeng, Yang.
  • Runfeng L; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Yunlong H; Hebei University of Chinese Medicine, College of Integrated Chinese and Western Medicine, China.
  • Jicheng H; Technology Centre, Guangzhou Customs, China.
  • Weiqi P; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Qinhai M; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Yongxia S; Technology Centre, Guangzhou Customs, China.
  • Chufang L; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Jin Z; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Zhenhua J; Hebei University of Chinese Medicine, College of Integrated Chinese and Western Medicine, China.
  • Haiming J; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Kui Z; Technology Centre, Guangzhou Customs, China.
  • Shuxiang H; Technology Centre, Guangzhou Customs, China.
  • Jun D; Technology Centre, Guangzhou Customs, China.
  • Xiaobo L; Technology Centre, Guangzhou Customs, China.
  • Xiaotao H; KingMed Virology Diagnostic & Translational Center, China.
  • Lin W; KingMed Virology Diagnostic & Translational Center, China.
  • Nanshan Z; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
  • Zifeng Y; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China; State Key Laboratory of Quality Research in Chinese Medicine,
Pharmacol Res ; 156: 104761, 2020 06.
Article in English | MEDLINE | ID: covidwho-830796
ABSTRACT

PURPOSE:

Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response.

METHODS:

The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays.

RESULTS:

LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells.

CONCLUSIONS:

LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Drugs, Chinese Herbal / Betacoronavirus / Anti-Inflammatory Agents Topics: Traditional medicine Limits: Animals Language: English Journal: Pharmacol Res Journal subject: Pharmacology Year: 2020 Document Type: Article Affiliation country: J.phrs.2020.104761

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Drugs, Chinese Herbal / Betacoronavirus / Anti-Inflammatory Agents Topics: Traditional medicine Limits: Animals Language: English Journal: Pharmacol Res Journal subject: Pharmacology Year: 2020 Document Type: Article Affiliation country: J.phrs.2020.104761