Immunohistochemical diagnosis method in parvovirus

ABSTRACT

10 cases of histopathologically suggestive lesions for parvovirus in the canine species were examined by the avidin-biotin-peroxidase complex. For this, a mouse monoclonal antibody was used. Following the immunohistochemical examination, positive results were obtained in 80% (8 dogs) of processed samples. The most frequent positive immunohistochemical reaction was observed in macrophages, lymphocytes, necrotic cells of the intestinal crypt, evidence of parvoviral antigen is reliable for determining the etiology of the disease if parvovirus is suspected on the basis of the lesions. In order to perform the diagnostic immunohistochemical procedure, the strepto-avidin-biotin-peroxidase method was used, using the monoclonal antibody. It is of murine origin. Negative cases of parvovirus by immunohistochemical examination show the existence of other causes that produce the same lesions;coronaviruses, rotaviruses or coronaviruses associated with clostridium perfringens which may cause intestinal necrosis of the crypt. All dog breeds can do this disease, but in the Poodle breed the disease has been more common. Sex is not a criterion of receptivity, but in proportion of over 60% were affected females. Regarding the seasonal evolution, the disease is more common in the winter period compared to the other seasons. © 2020 SYSCOM 18 S.R.L.. All rights reserved.