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Attenuated porcine-derived type 2 bovine viral diarrhea virus as vector stably expressing viral gene.
Tao, Jie; Li, Benqiang; Shi, Ying; Chen, Jinghua; Zhu, Guoqiang; Shen, Xiaohui; Liu, Huili.
  • Tao J; Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China.
  • Li B; Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China.
  • Shi Y; Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China.
  • Chen J; Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China.
  • Zhu G; College of Veterinary Medicine, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
  • Shen X; Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China.
  • Liu H; Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China. Electronic address: huili
J Virol Methods ; 279: 113842, 2020 05.
Article in English | MEDLINE | ID: covidwho-832023
ABSTRACT
Infectious bovine viral diarrhea virus (BVDV) cDNA clones have been used for the expression of classical swine fever virus (CSFV) genes for immune prevention and control. However, can it be used for the expression of an allogenetic fragment? To answer this question, a BVDV chimeric virus expressing the spike (S) antigen fragment of porcine epidemic diarrhea virus (PEDV) was constructed. Antigen S499-602 was inserted into pig-derived BVDV-2 infectious cDNA clone pASH28 in tandem by overlapping PCR, located between the seventh and eighth amino acids at the N-terminus of the capsid (C) protein of BVDV. Indirect immunofluorescence assay confirmed that the chimeric virus vASH-dS312 containing double S499-602 sequences was successfully assembled, which could react with the monoclonal antibody (MAb) against BVDV E2 and PEDV S proteins. Further western blot analysis confirmed that the exogenous S499-602 double protein could be stably expressed. Next, the chimeric virus vASH-dS312 was administered to BALB/C mice either orally or by intramuscular injection. The immunized mice were healthy and showed no signs of toxicity. IgG against BVDV and PEDV antibodies could be detected in the mice administered vASH-dS312 by intramuscular injection, which had neutralization activity against BVDV and PEDV. Thus, this study reported a new insertion site in the BVDV infectious cDNA clone that could successfully express an allogenetic antigen.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / Diarrhea Virus 2, Bovine Viral / Capsid Proteins / Porcine epidemic diarrhea virus / Antigens, Viral Topics: Vaccines Limits: Animals Language: English Journal: J Virol Methods Year: 2020 Document Type: Article Affiliation country: J.jviromet.2020.113842

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Vaccines / Diarrhea Virus 2, Bovine Viral / Capsid Proteins / Porcine epidemic diarrhea virus / Antigens, Viral Topics: Vaccines Limits: Animals Language: English Journal: J Virol Methods Year: 2020 Document Type: Article Affiliation country: J.jviromet.2020.113842