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An emerging novel bovine coronavirus with a 4-amino-acid insertion in the receptor-binding domain of the hemagglutinin-esterase gene.
Abi, Keha-Mo; Zhang, Qi; Zhang, Bin; Zhou, Long; Yue, Hua; Tang, Cheng.
  • Abi KM; College of Life Science and Technology, Southwest Minzu University, Chengdu, China.
  • Zhang Q; College of Life Science and Technology, Southwest Minzu University, Chengdu, China.
  • Zhang B; College of Life Science and Technology, Southwest Minzu University, Chengdu, China.
  • Zhou L; Key Laboratory of Qinghai Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, 610041, China.
  • Yue H; College of Life Science and Technology, Southwest Minzu University, Chengdu, China.
  • Tang C; College of Life Science and Technology, Southwest Minzu University, Chengdu, China. yhua900@163.com.
Arch Virol ; 165(12): 3011-3015, 2020 Dec.
Article in English | MEDLINE | ID: covidwho-833995
ABSTRACT
The hemagglutinin-esterase (HE) protein of betacoronavirus lineage A is a secondary receptor in the infection process and is involved in the emergence of new betacoronavirus genotypes with altered host specificity and tissue tropism. We previously reported a novel recombinant bovine coronavirus (BCoV) strain that was circulating in dairy cattle in China, but this virus was not successfully isolated, and the genetic characteristics of BCoV are still largely unknown. In this study, 20 diarrheic faecal samples were collected from a farm in Liaoning province that had an outbreak of calf diarrhea (≤ 3 months of age) in November 2018, and all of the samples tested positive for BCoV by RT-PCR. In addition, a BCoV strain with a recombinant HE (designated as SWUN/A1/2018) and another BCoV strain with a recombinant HE containing an insertion (designated as SWUN/A10/2018) were successfully isolated in cell culture (TCID50 104.25/mL and 104.73/mL, respectively). Unexpectedly, we identified the emergence of a novel BCoV variant characterized by a 12-nt bovine gene insertion in the receptor-binding domain in a natural recombinant HE gene, suggesting a novel evolutionary pattern in BCoV.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Cattle Diseases / Viral Fusion Proteins / Coronavirus, Bovine / Coronavirus Infections / Diarrhea / Hemagglutinins, Viral Type of study: Observational study / Randomized controlled trials Topics: Variants Limits: Animals Country/Region as subject: Asia Language: English Journal: Arch Virol Year: 2020 Document Type: Article Affiliation country: S00705-020-04840-y

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Cattle Diseases / Viral Fusion Proteins / Coronavirus, Bovine / Coronavirus Infections / Diarrhea / Hemagglutinins, Viral Type of study: Observational study / Randomized controlled trials Topics: Variants Limits: Animals Country/Region as subject: Asia Language: English Journal: Arch Virol Year: 2020 Document Type: Article Affiliation country: S00705-020-04840-y