Recent advances in the nucleic acid-based diagnostic tool for coronavirus.
Mol Biol Rep
; 47(11): 9033-9041, 2020 Nov.
Article
in English
| MEDLINE | ID: covidwho-834020
ABSTRACT
Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. Nucleic acid based detection tool for SARS-CoV-2.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
RNA, Viral
/
Coronavirus Infections
/
Nucleic Acid Amplification Techniques
/
Molecular Diagnostic Techniques
/
Betacoronavirus
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Mol Biol Rep
Year:
2020
Document Type:
Article
Affiliation country:
S11033-020-05889-3
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