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High stability of plant-expressed virus-like particles of an insect virus in artificial gastric and intestinal fluids.
Berardi, Alberto; Castells-Graells, Roger; Lomonossoff, George P.
  • Berardi A; Department of Pharmaceutical Sciences and Pharmaceutics, Faculty of Pharmacy, Applied Science Private University, Amman 11931, Jordan; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: a_berardi@asu.edu.jo.
  • Castells-Graells R; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
  • Lomonossoff GP; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Eur J Pharm Biopharm ; 155: 103-111, 2020 Oct.
Article in English | MEDLINE | ID: covidwho-837756
ABSTRACT
The harsh conditions of the gastro-intestinal (GI) milieu pose a major barrier to the oral delivery of protein nanocages. Here we studied the stability of Nudaurelia capensis omega virus (NωV) virus-like particles (VLPs) in simulated GI fluids. NωV VLPs capsids and procapsids were transiently expressed in plants, the VLPs were incubated in various simulated GI fluids and their stability was determined by gel electrophoresis, density gradient ultracentrifugation and transmission electron microscopy (TEM). The results showed that the capsids were highly resistant to simulated gastric fluids at pH ≥ 3. Even under the harshest conditions, which consisted of a pepsin solution at pH 1.2, NωV capsids remained assembled as VLPs, though some digestion of the coat protein occurred. Moreover, 80.8% (±10.2%) stability was measured for NωV capsids upon 4 h incubation in simulated intestinal fluids. The high resistance of this protein cage to digestion and denaturation can be attributed to its distinctively compact structure. The more porous form of the VLPs, the procapsid, was less stable under all conditions. Our results suggest that NωV VLPs capsids are likely to endure transit through the GI tract, designating them as promising candidate protein nanocages for oral drug delivery.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plants / RNA Viruses / Capsid / Nanoparticles / Insect Viruses Limits: Animals / Humans Language: English Journal: Eur J Pharm Biopharm Journal subject: Pharmacy / Pharmacology Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plants / RNA Viruses / Capsid / Nanoparticles / Insect Viruses Limits: Animals / Humans Language: English Journal: Eur J Pharm Biopharm Journal subject: Pharmacy / Pharmacology Year: 2020 Document Type: Article