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Whole blood immunophenotyping uncovers immature neutrophil-to-VD2 T-cell ratio as an early marker for severe COVID-19.
Carissimo, Guillaume; Xu, Weili; Kwok, Immanuel; Abdad, Mohammad Yazid; Chan, Yi-Hao; Fong, Siew-Wai; Puan, Kia Joo; Lee, Cheryl Yi-Pin; Yeo, Nicholas Kim-Wah; Amrun, Siti Naqiah; Chee, Rhonda Sin-Ling; How, Wilson; Chan, Stephrene; Fan, Bingwen Eugene; Andiappan, Anand Kumar; Lee, Bernett; Rötzschke, Olaf; Young, Barnaby Edward; Leo, Yee-Sin; Lye, David Chien; Renia, Laurent; Ng, Lai Guan; Larbi, Anis; Ng, Lisa Fp.
  • Carissimo G; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore. guillaume_carissimo@immunol.a-star.edu.sg.
  • Xu W; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore. guillaume_carissimo@immunol.a-star.edu.sg.
  • Kwok I; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Abdad MY; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Chan YH; National Centre for Infectious Diseases, 16 Jalan Tan Tock Seng, 308442, Singapore, Singapore.
  • Fong SW; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Puan KJ; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Lee CY; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Yeo NK; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Amrun SN; Department of Biological Sciences, National University of Singapore, Singapore, Singapore, 117543.
  • Chee RS; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • How W; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Chan S; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Fan BE; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Andiappan AK; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Lee B; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Rötzschke O; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Young BE; Infectious Disease Horizontal Technology Center, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Leo YS; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Lye DC; Singapore Immunology Network, Agency for Science, Technology and Research, Immunos, Biopolis, 138648, Singapore.
  • Renia L; Department of Haematology, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, 308433, Singapore, Singapore.
  • Ng LG; Department of Laboratory Medicine, Khoo Teck Puat Hospital, Singapore, Singapore.
  • Larbi A; Lee Kong Chian School of Medicine, Singapore, Singapore.
  • Ng LF; Yong Loo Lin School of Medicine, Singapore, Singapore.
Nat Commun ; 11(1): 5243, 2020 10 16.
Article in English | MEDLINE | ID: covidwho-872700
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
SARS-CoV-2 is the novel coronavirus responsible for the current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but the cellular mechanisms underlying this progression are still unknown. Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity. It would thus be a useful prognostic marker for preventive patient management and improved healthcare resource management.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Receptors, Antigen, T-Cell, gamma-delta / CD8-Positive T-Lymphocytes / Betacoronavirus / Neutrophils Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2020 Document Type: Article Affiliation country: S41467-020-19080-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Receptors, Antigen, T-Cell, gamma-delta / CD8-Positive T-Lymphocytes / Betacoronavirus / Neutrophils Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2020 Document Type: Article Affiliation country: S41467-020-19080-6