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A point-of-care selenium nanoparticle-based test for the combined detection of anti-SARS-CoV-2 IgM and IgG in human serum and blood.
Wang, Zhizeng; Zheng, Zhi; Hu, Hangzhan; Zhou, Qianwen; Liu, Wei; Li, Xiaoquan; Liu, Zhigang; Wang, Yaohui; Ma, Yuanfang.
  • Wang Z; Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China. wangyaohui2017@henu.edu.cn mayf@henu.edu.cn.
  • Zheng Z; Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China. wangyaohui2017@henu.edu.cn mayf@henu.edu.cn.
  • Hu H; Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China. wangyaohui2017@henu.edu.cn mayf@henu.edu.cn.
  • Zhou Q; Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China. wangyaohui2017@henu.edu.cn mayf@henu.edu.cn.
  • Liu W; The Institute for Disease Prevention and Control of People's Liberation Army, Beijing, 100071, China.
  • Li X; The First Affiliated Hospital of Henan University, Kaifeng, 475004, China.
  • Liu Z; The First Affiliated Hospital of Henan University, Kaifeng, 475004, China.
  • Wang Y; Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China. wangyaohui2017@henu.edu.cn mayf@henu.edu.cn.
  • Ma Y; Joint National Laboratory for Antibody Drug Engineering, Key Laboratory of Cellular and Molecular Immunology of Henan Province, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China. wangyaohui2017@henu.edu.cn mayf@henu.edu.cn.
Lab Chip ; 20(22): 4255-4261, 2020 11 10.
Article in English | MEDLINE | ID: covidwho-872742
ABSTRACT
COVID-19 is a widespread and highly contagious disease in the human population. COVID-19 is caused by SARS-CoV-2 infection. There is still a great demand for point-of-care tests for detection, epidemic prevention and epidemiological investigation, both now and after the epidemic. We present a lateral flow immunoassay kit based on a selenium nanoparticle-modified SARS-CoV-2 nucleoprotein, which detects anti-SARS-CoV-2 IgM and anti-SARS-CoV-2 IgG in human serum, and the results can be read by the naked eye in 10 minutes. We expressed and purified the SARS-CoV-2 nucleoprotein in HEK293 cells, with a purity of 98.14% and a concentration of 5 mg mL-1. Selenium nanoparticles were synthesized by l-ascorbic acid reduction of seleninic acid at room temperature. After conjugation with the nucleoprotein, a lateral flow kit was successfully prepared. The IgM and IgG detection limits of the lateral flow kit reached 20 ng mL-1 and 5 ng mL-1, respectively, in human serum. A clinical study sample comprising 90 COVID-19-diagnosed patients and 263 non-infected controls was used to demonstrate a sensitivity and specificity of 93.33% and 97.34%, respectively, based on RT-PCR and clinical results. No cross-reactions with rheumatoid factor and positive serum for anti-nuclear antibodies, influenza A, and influenza B were observed. Moreover, the lateral flow kit remained stable after storage for 30 days at 37 °C. Our results demonstrate that the selenium nanoparticle lateral flow kit can conveniently, rapidly, and sensitively detect anti-SARS-CoV-2 IgM and IgG in human serum and blood; it can also be suitable for the epidemiological investigation of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoglobulin G / Immunoglobulin M / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Lab Chip Journal subject: Biotechnology / Chemistry Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / Immunoglobulin G / Immunoglobulin M / Coronavirus Infections / Clinical Laboratory Techniques / Betacoronavirus / Antibodies, Viral Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Lab Chip Journal subject: Biotechnology / Chemistry Year: 2020 Document Type: Article