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Bacillus Calmette-Guérin (BCG) vaccine generates immunoregulatory cells in the cervical lymph nodes in guinea pigs injected intra dermally.
Vergkizi, Souzan; Nikolakakis, Ioannis.
  • Vergkizi S; Department of Microbiology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: sverg@auth.gr.
  • Nikolakakis I; Department of Pharmaceutical Technology, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: yannikos@pharm.auth.gr.
Vaccine ; 38(48): 7629-7637, 2020 11 10.
Article in English | MEDLINE | ID: covidwho-879777
ABSTRACT
This work demonstrates the presence of immune regulatory cells in the cervical lymph nodes draining Bacillus Calmette-Guérin (BCG) vaccinated site on the dorsum of the ear in guinea pigs. It is shown that whole cervical lymph node cells did not proliferate in vitro in the presence of soluble mycobacterial antigens (PPD or leprosin) despite being responsive to whole mycobacteria. Besides, T cells from these lymph nodes separated as a non-adherent fraction on a nylon wool column, proliferated to PPD in the presence of autologous antigen presenting cells. Interestingly, addition of as low as 20% nylon wool adherent cells to these, sharply decreased the proliferation by 83%. Looking into what cells in the adherent fraction suppressed the proliferation, it was found that neither the T cell nor the macrophage enriched cell fractions of this population individually showed suppressive effect, indicating that their co-presence was necessary for the suppression. Since BCG induced granulomas resolve much faster than granulomas induced by other mycobacteria such as Mycobacterium leprae the present experimental findings add to the existing evidence that intradermal BCG vaccination influences subsequent immune responses in the host and may further stress upon its beneficial role seen in Covid-19 patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: BCG Vaccine / T-Lymphocytes / Granuloma / Lymph Nodes / Antigens, Bacterial Topics: Vaccines Limits: Animals / Female / Humans / Male Language: English Journal: Vaccine Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: BCG Vaccine / T-Lymphocytes / Granuloma / Lymph Nodes / Antigens, Bacterial Topics: Vaccines Limits: Animals / Female / Humans / Male Language: English Journal: Vaccine Year: 2020 Document Type: Article