Neuropilin-1 is a host factor for SARS-CoV-2 infection.
Science
; 370(6518): 861-865, 2020 11 13.
Article
in English
| MEDLINE | ID: covidwho-883300
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), uses the viral spike (S) protein for host cell attachment and entry. The host protease furin cleaves the full-length precursor S glycoprotein into two associated polypeptides S1 and S2. Cleavage of S generates a polybasic Arg-Arg-Ala-Arg carboxyl-terminal sequence on S1, which conforms to a C-end rule (CendR) motif that binds to cell surface neuropilin-1 (NRP1) and NRP2 receptors. We used x-ray crystallography and biochemical approaches to show that the S1 CendR motif directly bound NRP1. Blocking this interaction by RNA interference or selective inhibitors reduced SARS-CoV-2 entry and infectivity in cell culture. NRP1 thus serves as a host factor for SARS-CoV-2 infection and may potentially provide a therapeutic target for COVID-19.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Neuropilin-1
/
Virus Internalization
/
Spike Glycoprotein, Coronavirus
/
Betacoronavirus
Limits:
Humans
Language:
English
Journal:
Science
Year:
2020
Document Type:
Article
Affiliation country:
Science.abd3072
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