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Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate.
Bangaru, Sandhya; Ozorowski, Gabriel; Turner, Hannah L; Antanasijevic, Aleksandar; Huang, Deli; Wang, Xiaoning; Torres, Jonathan L; Diedrich, Jolene K; Tian, Jing-Hui; Portnoff, Alyse D; Patel, Nita; Massare, Michael J; Yates, John R; Nemazee, David; Paulson, James C; Glenn, Greg; Smith, Gale; Ward, Andrew B.
  • Bangaru S; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Ozorowski G; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Turner HL; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Antanasijevic A; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Huang D; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Wang X; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Torres JL; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Diedrich JK; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Tian JH; Novavax, Inc., 21 Firstfield Road, Gaithersburg, MD 20878, USA.
  • Portnoff AD; Novavax, Inc., 21 Firstfield Road, Gaithersburg, MD 20878, USA.
  • Patel N; Novavax, Inc., 21 Firstfield Road, Gaithersburg, MD 20878, USA.
  • Massare MJ; Novavax, Inc., 21 Firstfield Road, Gaithersburg, MD 20878, USA.
  • Yates JR; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Nemazee D; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Paulson JC; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Glenn G; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
  • Smith G; Novavax, Inc., 21 Firstfield Road, Gaithersburg, MD 20878, USA.
  • Ward AB; Novavax, Inc., 21 Firstfield Road, Gaithersburg, MD 20878, USA.
Science ; 370(6520): 1089-1094, 2020 11 27.
Article in English | MEDLINE | ID: covidwho-883301
Preprint
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ABSTRACT
Vaccine efforts to combat the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is responsible for the current coronavirus disease 2019 (COVID-19) pandemic, are focused on SARS-CoV-2 spike glycoprotein, the primary target for neutralizing antibodies. We performed cryo-election microscopy and site-specific glycan analysis of one of the leading subunit vaccine candidates from Novavax, which is based on a full-length spike protein formulated in polysorbate 80 detergent. Our studies reveal a stable prefusion conformation of the spike immunogen with slight differences in the S1 subunit compared with published spike ectodomain structures. We also observed interactions between the spike trimers, allowing formation of higher-order spike complexes. This study confirms the structural integrity of the full-length spike protein immunogen and provides a basis for interpreting immune responses to this multivalent nanoparticle immunogen.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Vaccines Topics: Vaccines Limits: Humans Language: English Journal: Science Year: 2020 Document Type: Article Affiliation country: Science.abe1502

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Vaccines Topics: Vaccines Limits: Humans Language: English Journal: Science Year: 2020 Document Type: Article Affiliation country: Science.abe1502