Your browser doesn't support javascript.
A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2.
Lau, Yee Ling; Ismail, Ilyiana Binti; Izati Binti Mustapa, Nur; Lai, Meng Yee; Tuan Soh, Tuan Suhaila; Hassan, Afifah Haji; Peariasamy, Kalaiarasu M; Lee, Yee Leng; Goh, Pik Pin.
  • Lau YL; 1Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
  • Ismail IB; 2Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.
  • Izati Binti Mustapa N; 2Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.
  • Lai MY; 1Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
  • Tuan Soh TS; 2Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.
  • Hassan AH; 2Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.
  • Peariasamy KM; 3Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia.
  • Lee YL; 3Clinical Research Centre, Hospital Sungai Buloh, Selangor, Malaysia.
  • Goh PP; 4Institute for Clinical Research (ICR), National Institutes of Health (NIH), Ministry of Health Malaysia, Putrajaya, Malaysia.
Am J Trop Med Hyg ; 103(6): 2350-2352, 2020 Dec.
Article in English | MEDLINE | ID: covidwho-887652
ABSTRACT
A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Pandemics / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Am J Trop Med Hyg Year: 2020 Document Type: Article Affiliation country: Ajtmh.20-1079

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Pandemics / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Humans Language: English Journal: Am J Trop Med Hyg Year: 2020 Document Type: Article Affiliation country: Ajtmh.20-1079