Transcriptomic analysis reveals novel mechanisms of SARS-CoV-2 infection in human lung cells.
Immun Inflamm Dis
; 8(4): 753-762, 2020 12.
Article
in English
| MEDLINE | ID: covidwho-893223
ABSTRACT
BACKGROUND:
Severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) is a single-stranded RNA virus responsible for the global pandemic of the coronavirus disease-2019 (COVID-19). To date, there are still no effective approaches for the prevention and treatment of COVID-19.OBJECTIVE:
The present study aims to explore the possible mechanisms of SARS-CoV-2 infection in human lung cells.METHODS:
Data interpretation was conducted by recruiting bioinformatics analysis, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways analysis using downloaded data from the NCBI Gene Expression Omnibus database.RESULTS:
The present study demonstrated that SARS-CoV-2 infection induces the upregulation of 14 interferon-stimulated genes, indicative of immune, and interferon responses to the virus. Notably, genes for pyrimidine metabolism and steroid hormone biosynthesis are selectively enriched in human lung cells after SARS-CoV-2 infection, suggesting that altered pyrimidine metabolism and steroid biosynthesis are remarkable, and perhaps druggable features after SARS-CoV-2 infection. Besides, there is a strong positive correlation between viral ORF1ab, ORF6, and angiotensin-converting enzyme 2 (ACE2) expression in human lung cells, implying that ACE2 facilitates SARS-CoV-2 infection and replication in host cells probably through the induction of ORF1ab and ORF6.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
Interferons
/
Coronavirus Infections
/
Peptidyl-Dipeptidase A
/
Betacoronavirus
/
Lung
Limits:
Humans
Language:
English
Journal:
Immun Inflamm Dis
Year:
2020
Document Type:
Article
Affiliation country:
Iid3.366
Similar
MEDLINE
...
LILACS
LIS