Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using ß-propiolactone-inactivated, whole virus-based indirect ELISA.
J Virol Methods
; 287: 113996, 2021 01.
Article
in English
| MEDLINE | ID: covidwho-894099
ABSTRACT
BACKGROUND:
Coronavirus disease 2019 (COVID-19) pandemic caused by infection with severe acute respiratory syndrome - coronavirus-2 (SARS-CoV-2) continues to affect many countries and large populations. Serologic assays for antibody detection aid patient diagnosis and seroepidemiologic investigations.METHODS:
An indirect IgG ELISA was developed indigenously using ß-propiolactone (BPL) inactivated SARS-CoV-2. This assay was used for screening 200 healthy donor sera collected prior to COVID-19 emergence (2017-2019), 185 serum/plasma samples of confirmed COVID-19 patients (nâ¯=â¯137) and 57 samples of viral RNA positive asymptomatic contacts (nâ¯=â¯51). The IgG response was studied in relation to duration and severity of illness.RESULTS:
The ELISA demonstrated 97 % specificity and IgG detection in >50 %, 80 %, 93.8 % and 100 % of the patients respectively during the first, second, third and fourth week of illness. IgG detection rate was higher in patients with severe disease (SD, 90.9 %) than those with mild disease (MD, 68.8 %) during the second week of illness (Pâ¯=â¯0.027). IgG seropositivity among asymptomatic contacts was 64.7 %. IgG ELISA absorbance values were higher in SD than MD patients during the first 2 weeks of illness (Pâ¯<â¯0.05). No significant difference was observed between the absorbance values of asymptomatic subjects and MD patients (Pâ¯=â¯0.94).CONCLUSION:
The BPL inactivated virus-based ELISA could detect IgG antibodies early and in a significant proportion of COVID-19 patients suggesting its potential utility as a supplement to the currently used viral RNA detection tests in patient diagnosis and contact screening algorithms.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Propiolactone
/
Immunoglobulin G
/
COVID-19 Serological Testing
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
J Virol Methods
Year:
2021
Document Type:
Article
Affiliation country:
J.jviromet.2020.113996
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