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Hydroxychloroquine Exhibits a Strong Complex Interaction with DNA: Unraveling the Mechanism of Action.
Bazoni, R F; Moura, T A; Rocha, M S.
  • Bazoni RF; Departamento de Ciências Naturais, Universidade Federal do Espírito Santo, São Mateus, Espírito Santo 29.932-540, Brazil.
  • Moura TA; Departamento de Física, Universidade Federal de Viçosa. Viçosa, Minas Gerais 36.570-900, Brazil.
  • Rocha MS; Departamento de Física, Universidade Federal de Viçosa. Viçosa, Minas Gerais 36.570-900, Brazil.
J Phys Chem Lett ; 11(22): 9528-9534, 2020 Nov 19.
Article in English | MEDLINE | ID: covidwho-894363
ABSTRACT
In the past months, the use of the drug hydroxychloroquine has considerably increased in many countries, associated with a proposed treatment for the COVID-19 disease. Although there is no conclusive evidence about the efficacy of the drug for this purpose, surprisingly there are no conclusive studies in the literature concerning its mechanism of action inside cells, which is related to its interaction with nucleic acids. Here, we performed a robust characterization of the interaction between hydroxychloroquine and double-stranded DNA using single-molecule force spectroscopy and gel electrophoresis. Two different binding modes were identified, namely, minor groove binding for low drug concentrations and intercalation for high drug concentrations, and the sets of binding parameters were determined for each of these modes. Such results have unraveled in detail the molecular mechanism of action of the drug as a DNA ligand.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: DNA / Hydroxychloroquine / Intercalating Agents Language: English Journal: J Phys Chem Lett Year: 2020 Document Type: Article Affiliation country: Acs.jpclett.0c02590

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Full text: Available Collection: International databases Database: MEDLINE Main subject: DNA / Hydroxychloroquine / Intercalating Agents Language: English Journal: J Phys Chem Lett Year: 2020 Document Type: Article Affiliation country: Acs.jpclett.0c02590