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Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana.
Burimuah, Vitus; Sylverken, Augustina; Owusu, Michael; El-Duah, Philip; Yeboah, Richmond; Lamptey, Jones; Frimpong, Yaw Oppong; Agbenyega, Olivia; Folitse, Raphael; Emikpe, Ben; Tasiame, William; Owiredu, Eddie-Williams; Oppong, Samuel; Antwi, Christopher; Adu-Sarkodie, Yaw; Drosten, Christian.
  • Burimuah V; Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. vitus7uk@yahoo.co.uk.
  • Sylverken A; School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. vitus7uk@yahoo.co.uk.
  • Owusu M; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana. vitus7uk@yahoo.co.uk.
  • El-Duah P; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Yeboah R; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Lamptey J; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Frimpong YO; Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Agbenyega O; Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Folitse R; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Emikpe B; Institute of Virology, Universitätsmedizin Berlin, Charite, Germany.
  • Tasiame W; Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Owiredu EW; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Oppong S; Department of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Antwi C; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Adu-Sarkodie Y; Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana.
  • Drosten C; Department of Animal Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
BMC Vet Res ; 16(1): 405, 2020 Oct 27.
Article in English | MEDLINE | ID: covidwho-895005
Semantic information from SemMedBD (by NLM)
1. Bovine coronavirus PROCESS_OF Cattle
Subject
Bovine coronavirus
Predicate
PROCESS_OF
Object
Cattle
2. Bovine coronavirus PROCESS_OF Ruminants
Subject
Bovine coronavirus
Predicate
PROCESS_OF
Object
Ruminants
3. Bovine coronavirus COEXISTS_WITH Virus
Subject
Bovine coronavirus
Predicate
COEXISTS_WITH
Object
Virus
4. DNA Sequence PART_OF Capra hircus
Subject
DNA Sequence
Predicate
PART_OF
Object
Capra hircus
5. Bovine coronavirus PROCESS_OF Cattle
Subject
Bovine coronavirus
Predicate
PROCESS_OF
Object
Cattle
6. Bovine coronavirus PROCESS_OF Ruminants
Subject
Bovine coronavirus
Predicate
PROCESS_OF
Object
Ruminants
7. Bovine coronavirus COEXISTS_WITH Virus
Subject
Bovine coronavirus
Predicate
COEXISTS_WITH
Object
Virus
8. DNA Sequence PART_OF Capra hircus
Subject
DNA Sequence
Predicate
PART_OF
Object
Capra hircus
ABSTRACT

BACKGROUND:

Apart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to the livestock industry, particularly with respect to cattle rearing, continuous surveillance of the virus in cattle and small ruminants is essential in monitoring variations in the virus that could enhance host switching. In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide sequences were compared with homologous sequences from the GenBank.

RESULTS:

The study reports a BCoV prevalence of 0.3%, consisting of 4 positive cases; 3 goats and 1 cattle. Less than 10% of all the animals sampled showed clinical signs such as diarrhea and respiratory distress except for high temperature which occurred in > 1000 of the animals. However, none of the 4 BCoV positive animals manifested any clinical signs of the infection at the time of sample collection. Bayesian majority-rule cladogram comparing partial and full length BCoV RdRp genes obtained in the study to data from the GenBank revealed that the sequences obtained from this study formed one large monophyletic group with those from different species and countries. The goat sequences were similar to each other and clustered within the same clade. No major variations were thus observed between our isolates and those from elsewhere.

CONCLUSIONS:

Given that Ghana predominantly practices the extensive and semi-intensive systems of animal rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings with mixed husbandry and limited separation between species.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Sheep Diseases / Cattle Diseases / Goat Diseases / Coronavirus, Bovine / Coronavirus Infections Type of study: Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Animals Country/Region as subject: Africa Language: English Journal: BMC Vet Res Journal subject: Veterinary Medicine Year: 2020 Document Type: Article Affiliation country: S12917-020-02606-x

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Sheep Diseases / Cattle Diseases / Goat Diseases / Coronavirus, Bovine / Coronavirus Infections Type of study: Experimental Studies / Observational study / Prognostic study / Randomized controlled trials Limits: Animals Country/Region as subject: Africa Language: English Journal: BMC Vet Res Journal subject: Veterinary Medicine Year: 2020 Document Type: Article Affiliation country: S12917-020-02606-x